A Solid Phase Vibrational Circular Dichroism Study of Polypeptide-Surfactant Interaction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F15%3A43900287" target="_blank" >RIV/60461373:22340/15:43900287 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/chir.22534/abstract" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/chir.22534/abstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/chir.22534" target="_blank" >10.1002/chir.22534</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A Solid Phase Vibrational Circular Dichroism Study of Polypeptide-Surfactant Interaction

Popis výsledku v původním jazyce

We studied the interaction of poly-l-lysine (PLL) and poly-l-arginine (PLAG) with sodium dodecyl sulfate (SDS) surfactant and the interaction of poly-l-glutamic acid (PLGA) and poly-l-aspartic acid (PLAA) with tetradecyltrimethylammonium bromide (TTAB) surfactant using vibrational circular dichroism (VCD) spectroscopy in the region of C-H stretching vibration and in the Amide I region both in solution and in mulls. A chirality transfer from polypeptides to achiral surfactants was observed in the C-H stretching region, where measurements in solution were impossible. This observation was enabled by a special sample treatment technique using lyophilization and the preparation of mulls. This technique demonstrated itself as an interesting and beneficial tool for VCD measurements. In addition, we observed that SDS changed the secondary structure of PLL to the -sheet and of PLAG to the -helix. TTAB disrupted the PLGA and PLAA structure. These results were obtained in the mull but were confir

Název v anglickém jazyce

A Solid Phase Vibrational Circular Dichroism Study of Polypeptide-Surfactant Interaction

Popis výsledku anglicky

We studied the interaction of poly-l-lysine (PLL) and poly-l-arginine (PLAG) with sodium dodecyl sulfate (SDS) surfactant and the interaction of poly-l-glutamic acid (PLGA) and poly-l-aspartic acid (PLAA) with tetradecyltrimethylammonium bromide (TTAB) surfactant using vibrational circular dichroism (VCD) spectroscopy in the region of C-H stretching vibration and in the Amide I region both in solution and in mulls. A chirality transfer from polypeptides to achiral surfactants was observed in the C-H stretching region, where measurements in solution were impossible. This observation was enabled by a special sample treatment technique using lyophilization and the preparation of mulls. This technique demonstrated itself as an interesting and beneficial tool for VCD measurements. In addition, we observed that SDS changed the secondary structure of PLL to the -sheet and of PLAG to the -helix. TTAB disrupted the PLGA and PLAA structure. These results were obtained in the mull but were confir

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

<a href="/cs/project/GAP208%2F11%2F0105" target="_blank" >GAP208/11/0105: Rozšíření metod vibrační optické aktivity pro oblast biomolekul</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chirality

ISSN

0899-0042

e-ISSN

—

Svazek periodika

27

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

965-972

Kód UT WoS článku

000364635600014

EID výsledku v databázi Scopus

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