Speciation analysis of selenium in human urine by liquid chromatography and inductively coupled plasma mass spectrometry for monitoring of selenium in body fluids

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F15%3A43900396" target="_blank" >RIV/60461373:22340/15:43900396 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/0.1080/09542299.2015.1107502" target="_blank" >http://dx.doi.org/0.1080/09542299.2015.1107502</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/09542299.2015.1107502" target="_blank" >10.1080/09542299.2015.1107502</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Speciation analysis of selenium in human urine by liquid chromatography and inductively coupled plasma mass spectrometry for monitoring of selenium in body fluids

Popis výsledku v původním jazyce

Speciation analysis of selenium metabolites in urine was performed using hyphenation of mixed ion-pair reversed-phase chromatography and inductively coupled plasma mass spectrometry. A chromatographic separation was performed with a C8 stationary phase and a mobile phase containing 2.5 mmol LMINUS SIGN 1 sodium butane-1-sulfonate, 8 mmol LMINUS SIGN 1 tetramethylammonium hydroxide, 4 mmol LMINUS SIGN 1 malonic acid, and 0.05% methanol, pH 3.0. Under this condition, the selenium species selenite, selenate, selenomethionine, selenoethionine, selenourea, trimethylselenonium, and Se-methylselenocysteine were successfully separated. Selenium determination was carried out by monitoring 80Se. The internal standard Ge was added into the mobile phase. The calibration was linear at least up to 100 ?g LMINUS SIGN 1 Se for all species. The limit of detection was 0.4 ?g LMINUS SIGN 1 Se. When higher (1400 W) ICP power was applied, the calibration based solely on selenate provided accurate results f

Název v anglickém jazyce

Speciation analysis of selenium in human urine by liquid chromatography and inductively coupled plasma mass spectrometry for monitoring of selenium in body fluids

Popis výsledku anglicky

Speciation analysis of selenium metabolites in urine was performed using hyphenation of mixed ion-pair reversed-phase chromatography and inductively coupled plasma mass spectrometry. A chromatographic separation was performed with a C8 stationary phase and a mobile phase containing 2.5 mmol LMINUS SIGN 1 sodium butane-1-sulfonate, 8 mmol LMINUS SIGN 1 tetramethylammonium hydroxide, 4 mmol LMINUS SIGN 1 malonic acid, and 0.05% methanol, pH 3.0. Under this condition, the selenium species selenite, selenate, selenomethionine, selenoethionine, selenourea, trimethylselenonium, and Se-methylselenocysteine were successfully separated. Selenium determination was carried out by monitoring 80Se. The internal standard Ge was added into the mobile phase. The calibration was linear at least up to 100 ?g LMINUS SIGN 1 Se for all species. The limit of detection was 0.4 ?g LMINUS SIGN 1 Se. When higher (1400 W) ICP power was applied, the calibration based solely on selenate provided accurate results f

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemical Speciation and Bioavailability

ISSN

0954-2299

e-ISSN

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Svazek periodika

27

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

12

Strana od-do

127-138

Kód UT WoS článku

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EID výsledku v databázi Scopus

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