Experimental and CFD physical characterization of animal cell bioreactors: From micro- to production scale

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F18%3A43916534" target="_blank" >RIV/60461373:22340/18:43916534 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S1369703X17303388?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1369703X17303388?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bej.2017.12.004" target="_blank" >10.1016/j.bej.2017.12.004</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Experimental and CFD physical characterization of animal cell bioreactors: From micro- to production scale

Popis výsledku v původním jazyce

Transfer of mammalian cell culture processes across stirred and aerated bioreactor scales is a delicate task, frequently leading to different conditions across scales. To provide a more rational reasoning behind scale-up of mammalian cell cultures, physical characterization of bioreactors with working volumes of 15 mL, 3 L, 270 L, 5′000 L and 15′000 L was carried out using a combination of computational and experimental methods. Maximum hydrodynamic stress, mixing time and oxygen mass transfer coefficients were experimentally determined for all bioreactor scales. Computational fluid dynamic (CFD) simulations based on Reynolds-averaged Navier‐Stokes equation coupled with bubble size population balance equations were used to determine local as well as average hydrodynamic stresses and mass transfer coefficients. Furthermore, mixing times were determined by simulated tracer experiments. All calculations are well in agreement with experimentally measured values, thereby providing a validation of the CFD simulations. This integrated experimental and modeling methodology represents a valuable tool for a Quality-by-Design approach enabling the transfer of mammalian cell cultures in-between reactor scales, even for geometrically different reactors. Additionally, this study provides a rational framework to transfer an operating space developed at small scale to larger scales. © 2017 Elsevier B.V.

Název v anglickém jazyce

Experimental and CFD physical characterization of animal cell bioreactors: From micro- to production scale

Popis výsledku anglicky

Transfer of mammalian cell culture processes across stirred and aerated bioreactor scales is a delicate task, frequently leading to different conditions across scales. To provide a more rational reasoning behind scale-up of mammalian cell cultures, physical characterization of bioreactors with working volumes of 15 mL, 3 L, 270 L, 5′000 L and 15′000 L was carried out using a combination of computational and experimental methods. Maximum hydrodynamic stress, mixing time and oxygen mass transfer coefficients were experimentally determined for all bioreactor scales. Computational fluid dynamic (CFD) simulations based on Reynolds-averaged Navier‐Stokes equation coupled with bubble size population balance equations were used to determine local as well as average hydrodynamic stresses and mass transfer coefficients. Furthermore, mixing times were determined by simulated tracer experiments. All calculations are well in agreement with experimentally measured values, thereby providing a validation of the CFD simulations. This integrated experimental and modeling methodology represents a valuable tool for a Quality-by-Design approach enabling the transfer of mammalian cell cultures in-between reactor scales, even for geometrically different reactors. Additionally, this study provides a rational framework to transfer an operating space developed at small scale to larger scales. © 2017 Elsevier B.V.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20401 - Chemical engineering (plants, products)

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochemical Engineering Journal

ISSN

1369-703X

e-ISSN

—

Svazek periodika

131

Číslo periodika v rámci svazku

Neuveden

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

84-94

Kód UT WoS článku

000426028600011

EID výsledku v databázi Scopus

2-s2.0-85041128222