Effect of physicochemical parameters on the stability and activity of garlic alliinase and its use for in-situ allicin synthesis
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F21%3A43922875" target="_blank" >RIV/60461373:22340/21:43922875 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/61388963:_____/21:00541549
Výsledek na webu
<a href="https://doi.org/10.1371/journal.pone.0248878" target="_blank" >https://doi.org/10.1371/journal.pone.0248878</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1371/journal.pone.0248878" target="_blank" >10.1371/journal.pone.0248878</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Effect of physicochemical parameters on the stability and activity of garlic alliinase and its use for in-situ allicin synthesis
Popis výsledku v původním jazyce
Garlic is a well-known example of natural self-defence system consisting of an inactive substrate (alliin) and enzyme (alliinase) which, when combined, produce highly antimicrobial allicin. Increase of alliinase stability and its activity are of paramount importance in various applications relying on its use for in-situ synthesis of allicin or its analogues, e.g., pulmonary drug delivery, treatment of superficial injuries, or urease inhibitors in fertilizers. Here, we discuss the effect of temperature, pH, buffers, salts, and additives, i.e. antioxidants, chelating agents, reducing agents and cosolvents, on the stability and the activity of alliinase extracted from garlic. The effects of the storage temperature and relative humidity on the stability of lyophilized alliinase was demonstrated. A combination of the short half-life, high reactivity and non-specificity to particular proteins are reasons most bacteria cannot deal with allicin's mode of action and develop effective defence mechanism, which could be the key to sustainable drug design addressing serious problems with escalating emergence of multidrug-resistant (MDR) bacterial strains. © 2021 Public Library of Science. All rights reserved.
Název v anglickém jazyce
Effect of physicochemical parameters on the stability and activity of garlic alliinase and its use for in-situ allicin synthesis
Popis výsledku anglicky
Garlic is a well-known example of natural self-defence system consisting of an inactive substrate (alliin) and enzyme (alliinase) which, when combined, produce highly antimicrobial allicin. Increase of alliinase stability and its activity are of paramount importance in various applications relying on its use for in-situ synthesis of allicin or its analogues, e.g., pulmonary drug delivery, treatment of superficial injuries, or urease inhibitors in fertilizers. Here, we discuss the effect of temperature, pH, buffers, salts, and additives, i.e. antioxidants, chelating agents, reducing agents and cosolvents, on the stability and the activity of alliinase extracted from garlic. The effects of the storage temperature and relative humidity on the stability of lyophilized alliinase was demonstrated. A combination of the short half-life, high reactivity and non-specificity to particular proteins are reasons most bacteria cannot deal with allicin's mode of action and develop effective defence mechanism, which could be the key to sustainable drug design addressing serious problems with escalating emergence of multidrug-resistant (MDR) bacterial strains. © 2021 Public Library of Science. All rights reserved.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
20401 - Chemical engineering (plants, products)
Návaznosti výsledku
Projekt
<a href="/cs/project/TJ01000313" target="_blank" >TJ01000313: Vývoj biomimetických částic pro antibakteriální aplikace</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Plos One
ISSN
1932-6203
e-ISSN
—
Svazek periodika
16
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
20
Strana od-do
"e0248878"
Kód UT WoS článku
000631030200005
EID výsledku v databázi Scopus
2-s2.0-85102896668