In vitro assessment of selenium bioavailability from selenized lactic acid bacteria using a static INFOGEST digestion model and intestinal permeability model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F25%3A43931434" target="_blank" >RIV/60461373:22340/25:43931434 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/25:102118 RIV/26722861:_____/25:N0000002

Výsledek na webu

<a href="https://doi.org/10.1016/j.jtemb.2025.127632" target="_blank" >https://doi.org/10.1016/j.jtemb.2025.127632</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jtemb.2025.127632" target="_blank" >10.1016/j.jtemb.2025.127632</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro assessment of selenium bioavailability from selenized lactic acid bacteria using a static INFOGEST digestion model and intestinal permeability model

Popis výsledku v původním jazyce

Background and objective: Microbial selenium (Se) supplementation is an essential area of biotechnologicalresearch due to differences in the bioavailability and toxicity of different forms of selenium. To date, research hasfocused mainly on the use of selenized yeast. However, in recent years, scientific interest has also increased inother microorganisms, such as lactic acid bacteria (LAB), which have several unique properties that can affect thequality and bioavailability of selenium. LAB, unlike yeast, can also act as probiotics, which may bring additionalhealth benefits related to improving the intestinal microbiota and supporting the health of the gastrointestinaltract.Methods: This study investigates the in vitro bioaccessibility and bioavailability of Se from two lactic acid bacterialstrains, Streptococcus thermophilus CCDM 144 and Enterococcus faecium CCDM 922 A. We evaluated Seaccumulation, speciation, and stability during simulated gastrointestinal digestion and Se permeation through aCaco-2 cell monolayer model.Results: Both strains accumulated Se, metabolizing it predominantly into selenium nanoparticles (SeNPs,64–77 % of total Se), with only a minor fraction (&lt;5 % of total Se) of organic Se species. Experiments revealedthat while organic Se species had high bioavailability (up to 90 %), their bioaccessibility during digestion wasvery low (&lt;0.1 % of total Se). In contrast, SeNPs showed high bioaccessibility (~90 %) and moderate transportefficiency through the intestinal model (16–19 % after 4 hours).Conclusion: These results highlight the potential of SeNPs produced by lactic acid bacteria as a bioaccessible formof Se for dietary supplementation. Further research is required to explore the behavior of SeNPs within thehuman body to fully understand how they can be used safely and effectively in nutrition or other applications.

Název v anglickém jazyce

In vitro assessment of selenium bioavailability from selenized lactic acid bacteria using a static INFOGEST digestion model and intestinal permeability model

Popis výsledku anglicky

Background and objective: Microbial selenium (Se) supplementation is an essential area of biotechnologicalresearch due to differences in the bioavailability and toxicity of different forms of selenium. To date, research hasfocused mainly on the use of selenized yeast. However, in recent years, scientific interest has also increased inother microorganisms, such as lactic acid bacteria (LAB), which have several unique properties that can affect thequality and bioavailability of selenium. LAB, unlike yeast, can also act as probiotics, which may bring additionalhealth benefits related to improving the intestinal microbiota and supporting the health of the gastrointestinaltract.Methods: This study investigates the in vitro bioaccessibility and bioavailability of Se from two lactic acid bacterialstrains, Streptococcus thermophilus CCDM 144 and Enterococcus faecium CCDM 922 A. We evaluated Seaccumulation, speciation, and stability during simulated gastrointestinal digestion and Se permeation through aCaco-2 cell monolayer model.Results: Both strains accumulated Se, metabolizing it predominantly into selenium nanoparticles (SeNPs,64–77 % of total Se), with only a minor fraction (&lt;5 % of total Se) of organic Se species. Experiments revealedthat while organic Se species had high bioavailability (up to 90 %), their bioaccessibility during digestion wasvery low (&lt;0.1 % of total Se). In contrast, SeNPs showed high bioaccessibility (~90 %) and moderate transportefficiency through the intestinal model (16–19 % after 4 hours).Conclusion: These results highlight the potential of SeNPs produced by lactic acid bacteria as a bioaccessible formof Se for dietary supplementation. Further research is required to explore the behavior of SeNPs within thehuman body to fully understand how they can be used safely and effectively in nutrition or other applications.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/QK22010186" target="_blank" >QK22010186: Postbiotika, bakteriální exopolysacharidy a nové oligosacharidy pro funkční synbiotické fermentované výrobky</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY

ISSN

0946-672X

e-ISSN

1878-3252

Svazek periodika

88

Číslo periodika v rámci svazku

April 2025

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

nestránkováno

Kód UT WoS článku

001445514400001

EID výsledku v databázi Scopus

2-s2.0-86000367372