Enterococcus faecium CCDM 922A as a source of organically bound selenium

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F26722861%3A_____%2F21%3AN0000067" target="_blank" >RIV/26722861:_____/21:N0000067 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Enterococcus faecium CCDM 922A as a source of organically bound selenium

Popis výsledku v původním jazyce

A concept of selenization of yeasts and lactic acid bacteria (LAB) is gaining popularity recently. This novel approach allows to combine the individual health effects of these beneficial microorganisms together with supplying selenium (Se) – as an essential microelement in a more bioavailable and less toxic form. It is known, that some yeasts and LAB are able to transform inorganic Se forms into organically bound forms and elemental Se when grown in a medium containing selenite. This study investigated the bioavailability of Se in the strain Enterococcus faecium (EF) CCDM 922A in a CD (SD-Sprague Dawley) IGS rat model. The strain has previously been enriched by Se in the form of sodium selenite (50 mg/L) which was added into the M17 cultivation medium. After incubation and cell separation, the inoculum of selenized bacterial suspension was added to ultra-het treated skimmed milk and fermented. Following this, the samples were lyophilized and Se-enriched biomass of the strain CCDM 922A was obtained and used for further application into foodstuffs. The diets were manufactured by Altromin Spezialfutter GmbH & Co. The experimental in vivo study was approved by the committee of the Ministry of Education, Youth and Sports of the Czech Republic (No. MSMT-11197/2020-2). A total of 32 adult CD IGS male rats (Velaz, Prague, Czech Republic) were used in this study. Six-week-old rats were randomly divided into 4 groups (A-D; n=8). Following acclimatization, the experimental diets were administered to the animals for 58 days. Group A was fed a standard maintenance diet of Altromin 1324, control group (B) was fed a Se-deficient diet of Altromin C1045, group C was fed Altromin C1045 with added Enterococcus faecium CCDM 922A and group D was fed Altromin C1045 with added selenized Enterococcus faecium CCDM 922A strain. Twenty-two grams of the appropriate diet was administered daily to the animals and after 58 days, rats were euthanized by decapitation following anesthetization. Serum and tissue samples of kidneys, liver, heart and brain were collected for further analyses. Total Se in liver and kidneys were determined using ICP-MS after microwave-assisted acid digestion and Se-species were identified using HPLC coupled with ICP-MS. Se-enriched strain CCDM 922A administration resulted in higher Se concentrations in tissue samples of kidneys and liver of rats, where selenocystine was the prevalent Se species, followed by selenomethionine. In kidney tissues, also Se-methylselenocysteine was detected. Moreover, the production of extracellular SeNPs of different sizes (42-185 nm) was confirmed by TEM-EDX. The administration of selenized Enterococcus faecium improved the antioxidant status of rats. The effect of the diet was more pronounced in the heart tissue, where a lower glutathione reductase content was detected. Also reduced concentrations of serum malondialdehyde were noted following Se-supplementation and rats fed diets with EF and SeEF had higher glutathione reductase activity. The experimental diets showed no macroscopic effects on visceral organs and had no apparent influence on the lipid metabolism parameters. The CCDM 922A strain showed potential as a promising source of organically bound Se and SeNPs. Citation: Mrvikova I., Krausova G., Kana A., Medova K., Hyrslova I., Vecka M., Kantorova V.: Enterococcus faecium CCDM 922A as a source of organically bound selenium. Poster, BIOTECHMEET, 2021, 16.-18.09.2021, on-line

Název v anglickém jazyce

Enterococcus faecium CCDM 922A as a source of organically bound selenium

Popis výsledku anglicky

A concept of selenization of yeasts and lactic acid bacteria (LAB) is gaining popularity recently. This novel approach allows to combine the individual health effects of these beneficial microorganisms together with supplying selenium (Se) – as an essential microelement in a more bioavailable and less toxic form. It is known, that some yeasts and LAB are able to transform inorganic Se forms into organically bound forms and elemental Se when grown in a medium containing selenite. This study investigated the bioavailability of Se in the strain Enterococcus faecium (EF) CCDM 922A in a CD (SD-Sprague Dawley) IGS rat model. The strain has previously been enriched by Se in the form of sodium selenite (50 mg/L) which was added into the M17 cultivation medium. After incubation and cell separation, the inoculum of selenized bacterial suspension was added to ultra-het treated skimmed milk and fermented. Following this, the samples were lyophilized and Se-enriched biomass of the strain CCDM 922A was obtained and used for further application into foodstuffs. The diets were manufactured by Altromin Spezialfutter GmbH & Co. The experimental in vivo study was approved by the committee of the Ministry of Education, Youth and Sports of the Czech Republic (No. MSMT-11197/2020-2). A total of 32 adult CD IGS male rats (Velaz, Prague, Czech Republic) were used in this study. Six-week-old rats were randomly divided into 4 groups (A-D; n=8). Following acclimatization, the experimental diets were administered to the animals for 58 days. Group A was fed a standard maintenance diet of Altromin 1324, control group (B) was fed a Se-deficient diet of Altromin C1045, group C was fed Altromin C1045 with added Enterococcus faecium CCDM 922A and group D was fed Altromin C1045 with added selenized Enterococcus faecium CCDM 922A strain. Twenty-two grams of the appropriate diet was administered daily to the animals and after 58 days, rats were euthanized by decapitation following anesthetization. Serum and tissue samples of kidneys, liver, heart and brain were collected for further analyses. Total Se in liver and kidneys were determined using ICP-MS after microwave-assisted acid digestion and Se-species were identified using HPLC coupled with ICP-MS. Se-enriched strain CCDM 922A administration resulted in higher Se concentrations in tissue samples of kidneys and liver of rats, where selenocystine was the prevalent Se species, followed by selenomethionine. In kidney tissues, also Se-methylselenocysteine was detected. Moreover, the production of extracellular SeNPs of different sizes (42-185 nm) was confirmed by TEM-EDX. The administration of selenized Enterococcus faecium improved the antioxidant status of rats. The effect of the diet was more pronounced in the heart tissue, where a lower glutathione reductase content was detected. Also reduced concentrations of serum malondialdehyde were noted following Se-supplementation and rats fed diets with EF and SeEF had higher glutathione reductase activity. The experimental diets showed no macroscopic effects on visceral organs and had no apparent influence on the lipid metabolism parameters. The CCDM 922A strain showed potential as a promising source of organically bound Se and SeNPs. Citation: Mrvikova I., Krausova G., Kana A., Medova K., Hyrslova I., Vecka M., Kantorova V.: Enterococcus faecium CCDM 922A as a source of organically bound selenium. Poster, BIOTECHMEET, 2021, 16.-18.09.2021, on-line

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

40401 - Agricultural biotechnology and food biotechnology

Projekt

<a href="/cs/project/LTC20014" target="_blank" >LTC20014: Research of selenium-enriched lactic acid bacteria, bifidobacteria and yeasts and influence of low-pH stress exposure on their selenium biotransformation efficiency.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů