GC/MS determination of ibotenic acid and muscimol in the urine of patients intoxicated with Amanita pantherina
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61383082%3A_____%2F11%3A0018" target="_blank" >RIV/61383082:_____/11:0018 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s00414-011-0599-9" target="_blank" >http://dx.doi.org/10.1007/s00414-011-0599-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00414-011-0599-9" target="_blank" >10.1007/s00414-011-0599-9</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
GC/MS determination of ibotenic acid and muscimol in the urine of patients intoxicated with Amanita pantherina
Popis výsledku v původním jazyce
Authors of this article introduce an instrumental method of proving Amanita pantherina or A. muscaria poisoning. The article describes the isolation of ibotenic acid and muscimol from urine, the derivatization step and the determination of these compounds by gas chromatography/mass spectrometry. Isolation of these alkaloids from urine was performed on a strong cation exchanger (Dowex? 50W X8), and the elution and derivatization of the alkaloids were made in one step with ethyl chloroformate in aqueous solution of sodium hydroxide with the addition of ethanol and pyridine. Cycloserine was used as internal standard. By this method, concentrations of ibotenic acid and muscimol in the urine of four persons intoxicated with Amanita pantherina were determined. In this study mass spectra of derivatized ibotenic acid and muscimol are shown and validation of the method is described.
Název v anglickém jazyce
GC/MS determination of ibotenic acid and muscimol in the urine of patients intoxicated with Amanita pantherina
Popis výsledku anglicky
Authors of this article introduce an instrumental method of proving Amanita pantherina or A. muscaria poisoning. The article describes the isolation of ibotenic acid and muscimol from urine, the derivatization step and the determination of these compounds by gas chromatography/mass spectrometry. Isolation of these alkaloids from urine was performed on a strong cation exchanger (Dowex? 50W X8), and the elution and derivatization of the alkaloids were made in one step with ethyl chloroformate in aqueous solution of sodium hydroxide with the addition of ethanol and pyridine. Cycloserine was used as internal standard. By this method, concentrations of ibotenic acid and muscimol in the urine of four persons intoxicated with Amanita pantherina were determined. In this study mass spectra of derivatized ibotenic acid and muscimol are shown and validation of the method is described.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
FP - Ostatní lékařské obory
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
International Journal of Legal Medicine
ISSN
1437-1596
e-ISSN
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Svazek periodika
2011
Číslo periodika v rámci svazku
7
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
6
Strana od-do
1-6
Kód UT WoS článku
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EID výsledku v databázi Scopus
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