RGDS - and doxorubicin-modified poly[N-(2-hydroxypropyl) methacrylamide]-coated gamma-Fe2O3 nanoparticles.
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61383082%3A_____%2F21%3A00001059" target="_blank" >RIV/61383082:_____/21:00001059 - isvavai.cz</a>
Výsledek na webu
<a href="https://link.springer.com/article/10.1007/s00396-021-04895-6" target="_blank" >https://link.springer.com/article/10.1007/s00396-021-04895-6</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00396-021-04895-6" target="_blank" >10.1007/s00396-021-04895-6</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
RGDS - and doxorubicin-modified poly[N-(2-hydroxypropyl) methacrylamide]-coated gamma-Fe2O3 nanoparticles.
Popis výsledku v původním jazyce
Block copolymer comprising of hydrophilic poly[N-(2-hydroxypropyl)methacrylamide] (PHP) and reactive poly[N-(2-hydrazinyl-2-oxoethyl)methacrylamide] (PMAH) was synthesized by a reversible addition-fragmentation chain transfer (RAFT) polymerization and conjugated with doxorubicin (Dox) and/or RGDS targeting peptide via one-step reaction using N-gamma-maleimidobutyryl-oxysuccinimide ester. The resulting copolymer served as a coating of magnetic gamma-Fe2O3 nanoparticles that were tested in cell proliferation and in vivo experiments on a mice model with inoculated rat C6 glioblastoma tumor. The nanoparticles conjugated with RGDS peptide and doxorubicin easily engulfed both C6 tumor cell line, primary glioblastoma (GB) cells, and human mesenchymal stem cells (hMSC) used as a control; the particles decreased the GB cell growth by 45% compared to control cells without any treatment. Moreover, the gamma-Fe2O3@P(HP-MAH)-RGDS-Dox nanoparticles injected into C6 glioblastoma cell-derived tumors grown in the posterior flank of mice decreased the tumor size and more apoptotic cells were spread compared to that treated with free Dox.
Název v anglickém jazyce
RGDS - and doxorubicin-modified poly[N-(2-hydroxypropyl) methacrylamide]-coated gamma-Fe2O3 nanoparticles.
Popis výsledku anglicky
Block copolymer comprising of hydrophilic poly[N-(2-hydroxypropyl)methacrylamide] (PHP) and reactive poly[N-(2-hydrazinyl-2-oxoethyl)methacrylamide] (PMAH) was synthesized by a reversible addition-fragmentation chain transfer (RAFT) polymerization and conjugated with doxorubicin (Dox) and/or RGDS targeting peptide via one-step reaction using N-gamma-maleimidobutyryl-oxysuccinimide ester. The resulting copolymer served as a coating of magnetic gamma-Fe2O3 nanoparticles that were tested in cell proliferation and in vivo experiments on a mice model with inoculated rat C6 glioblastoma tumor. The nanoparticles conjugated with RGDS peptide and doxorubicin easily engulfed both C6 tumor cell line, primary glioblastoma (GB) cells, and human mesenchymal stem cells (hMSC) used as a control; the particles decreased the GB cell growth by 45% compared to control cells without any treatment. Moreover, the gamma-Fe2O3@P(HP-MAH)-RGDS-Dox nanoparticles injected into C6 glioblastoma cell-derived tumors grown in the posterior flank of mice decreased the tumor size and more apoptotic cells were spread compared to that treated with free Dox.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30107 - Medicinal chemistry
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2021
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
COLLOID AND POLYMER SCIENCE
ISSN
0303-402X
e-ISSN
—
Svazek periodika
2021
Číslo periodika v rámci svazku
Oct2021
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
11
Strana od-do
1-11
Kód UT WoS článku
000702787800001
EID výsledku v databázi Scopus
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