RGDS- and doxorubicin-modified poly[N-(2-hydroxypropyl)methacrylamide]-coated .gamma.-Fe2O3 nanoparticles for treatment of glioblastoma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61389013%3A_____%2F22%3A00556055" target="_blank" >RIV/61389013:_____/22:00556055 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/22:00556055 RIV/00216208:11110/22:10432518 RIV/00216208:11130/22:10432518

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s00396-021-04895-6" target="_blank" >https://link.springer.com/article/10.1007/s00396-021-04895-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00396-021-04895-6" target="_blank" >10.1007/s00396-021-04895-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

RGDS- and doxorubicin-modified poly[N-(2-hydroxypropyl)methacrylamide]-coated .gamma.-Fe2O3 nanoparticles for treatment of glioblastoma

Popis výsledku v původním jazyce

Block copolymer comprising of hydrophilic poly[N-(2-hydroxypropyl)methacrylamide] (PHP) and reactive poly[N-(2-hydrazinyl-2-oxoethyl)methacrylamide] (PMAH) was synthesized by a reversible addition-fragmentation chain transfer (RAFT) polymerization and conjugated with doxorubicin (Dox) and/or RGDS targeting peptide via one-step reaction using N-γ-maleimidobutyryl-oxysuccinimide ester. The resulting copolymer served as a coating of magnetic γ-Fe2O3 nanoparticles that were tested in cell proliferation and in vivo experiments on a mice model with inoculated rat C6 glioblastoma tumor. The nanoparticles conjugated with RGDS peptide and doxorubicin easily engulfed both C6 tumor cell line, primary glioblastoma (GB) cells, and human mesenchymal stem cells (hMSC) used as a control. The particles decreased the GB cell growth by 45% compared to control cells without any treatment. Moreover, the γ-Fe2O3@P(HP-MAH)-RGDS-Dox nanoparticles injected into C6 glioblastoma cell-derived tumors grown in the posterior flank of mice decreased the tumor size and more apoptotic cells were spread compared to that treated with free Dox.

Název v anglickém jazyce

RGDS- and doxorubicin-modified poly[N-(2-hydroxypropyl)methacrylamide]-coated .gamma.-Fe2O3 nanoparticles for treatment of glioblastoma

Popis výsledku anglicky

Block copolymer comprising of hydrophilic poly[N-(2-hydroxypropyl)methacrylamide] (PHP) and reactive poly[N-(2-hydrazinyl-2-oxoethyl)methacrylamide] (PMAH) was synthesized by a reversible addition-fragmentation chain transfer (RAFT) polymerization and conjugated with doxorubicin (Dox) and/or RGDS targeting peptide via one-step reaction using N-γ-maleimidobutyryl-oxysuccinimide ester. The resulting copolymer served as a coating of magnetic γ-Fe2O3 nanoparticles that were tested in cell proliferation and in vivo experiments on a mice model with inoculated rat C6 glioblastoma tumor. The nanoparticles conjugated with RGDS peptide and doxorubicin easily engulfed both C6 tumor cell line, primary glioblastoma (GB) cells, and human mesenchymal stem cells (hMSC) used as a control. The particles decreased the GB cell growth by 45% compared to control cells without any treatment. Moreover, the γ-Fe2O3@P(HP-MAH)-RGDS-Dox nanoparticles injected into C6 glioblastoma cell-derived tumors grown in the posterior flank of mice decreased the tumor size and more apoptotic cells were spread compared to that treated with free Dox.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Colloid and Polymer Science

ISSN

0303-402X

e-ISSN

1435-1536

Svazek periodika

300

Číslo periodika v rámci svazku

4 SI

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

267-277

Kód UT WoS článku

000702787800001

EID výsledku v databázi Scopus

2-s2.0-85116257699