Assessment of colorectal anastomosis perfusion with confocal laser endomicroscopy − an experimental study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61383082%3A_____%2F22%3A00001259" target="_blank" >RIV/61383082:_____/22:00001259 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00023001:_____/22:00083544 RIV/00064165:_____/22:10455245 RIV/00216208:11110/22:10455245 RIV/00216208:11130/22:10455245

Výsledek na webu

<a href="https://www.medvik.cz/bmc/searchcl.do" target="_blank" >https://www.medvik.cz/bmc/searchcl.do</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.33699/PIS.2022.101.12.593-598" target="_blank" >10.33699/PIS.2022.101.12.593-598</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessment of colorectal anastomosis perfusion with confocal laser endomicroscopy − an experimental study

Popis výsledku v původním jazyce

Early diagnosis of complicated healing of colorectal anastomosis can increase the chance for salvage surgery and thus reduce overall morbidity. Confocal laser endomicroscopy (CLE) enables in vivo assessment of tissue perfusion without disturbing its integrity. This experimental study evaluates the potential of CLE for postoperative monitoring of colorectal anastomosis. Methods: A hand-sewn colorectal anastomosis was performed in 9 pigs. The animals were subsequently divided into groups with normal (N=3) and ischemic anastomosis (N=6). Microscopic signs of hypoperfusion were evaluated postoperatively at regular intervals using CLE. Results: Uneven saturation of the images was evident in the group with ischemic anastomosis. The epithelium had inhomogeneous edges and more numerous crypt branching was visible. Tissue oedema quantified as the number of crypts per visual field was already more extensive at the first measurement after induction of ischemia. There was also a significant difference between the values measured before and 10 minutes after ischemia – 8.7±1.9 vs. 6.0±1.1 (p=0.013). Conclusion: Postoperative monitoring of the colorectal anastomosis using CLE enables prompt detection of perfusion disorders.

Název v anglickém jazyce

Assessment of colorectal anastomosis perfusion with confocal laser endomicroscopy − an experimental study

Popis výsledku anglicky

Early diagnosis of complicated healing of colorectal anastomosis can increase the chance for salvage surgery and thus reduce overall morbidity. Confocal laser endomicroscopy (CLE) enables in vivo assessment of tissue perfusion without disturbing its integrity. This experimental study evaluates the potential of CLE for postoperative monitoring of colorectal anastomosis. Methods: A hand-sewn colorectal anastomosis was performed in 9 pigs. The animals were subsequently divided into groups with normal (N=3) and ischemic anastomosis (N=6). Microscopic signs of hypoperfusion were evaluated postoperatively at regular intervals using CLE. Results: Uneven saturation of the images was evident in the group with ischemic anastomosis. The epithelium had inhomogeneous edges and more numerous crypt branching was visible. Tissue oedema quantified as the number of crypts per visual field was already more extensive at the first measurement after induction of ischemia. There was also a significant difference between the values measured before and 10 minutes after ischemia – 8.7±1.9 vs. 6.0±1.1 (p=0.013). Conclusion: Postoperative monitoring of the colorectal anastomosis using CLE enables prompt detection of perfusion disorders.

Druh

J_SC - Článek v periodiku v databázi SCOPUS

CEP obor

—

OECD FORD obor

30212 - Surgery

Projekt

<a href="/cs/project/NV19-08-00449" target="_blank" >NV19-08-00449: Nové minimálně invazivní možnosti léčby pooperační recidivy Crohnovy choroby na experimentálním modelu.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Rozhledy v chirurgii

ISSN

0035-9351

e-ISSN

—

Svazek periodika

101

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

6

Strana od-do

593 - 598

Kód UT WoS článku

—

EID výsledku v databázi Scopus

2-s2.0-85147785384