Flow electrochemical biosensors based on enzymatic porous reactor and tubular detector of silver solid amalgam

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F13%3A00392185" target="_blank" >RIV/61388955:_____/13:00392185 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.aca.2013.03.055" target="_blank" >http://dx.doi.org/10.1016/j.aca.2013.03.055</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aca.2013.03.055" target="_blank" >10.1016/j.aca.2013.03.055</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Flow electrochemical biosensors based on enzymatic porous reactor and tubular detector of silver solid amalgam

Popis výsledku v původním jazyce

A flow amperometric enzymatic biosensor for the determination of glucose was constructed. The biosensor consists of a flow reactor based on porous silver solid amalgam (AgSA) and a flow tubular detector based on compact AgSA. The preparation of the sensor and the determination of glucose occurred in three steps. First, a self-assembled monolayer of 11-mercaptoundecanoic acid (MUA) was formed at the porous surface of the reactor. Second, enzyme glucose oxidase (GOx) was covalently immobilized at MUA-layer using N-ethyl-N-(3-dimethylaminopropyl) carboimide and N-hydroxysuccinimide chemistry. Finally, a decrease of oxygen concentration (directly proportional to the concentration of glucose) during enzymatic reaction was amperometrically measured on the tubular detector under flow injection conditions. The following parameters of glucose determination were optimized with respect to amperometric response: composition of the mobile phase, its concentration, the potential of detection and the

Název v anglickém jazyce

Flow electrochemical biosensors based on enzymatic porous reactor and tubular detector of silver solid amalgam

Popis výsledku anglicky

A flow amperometric enzymatic biosensor for the determination of glucose was constructed. The biosensor consists of a flow reactor based on porous silver solid amalgam (AgSA) and a flow tubular detector based on compact AgSA. The preparation of the sensor and the determination of glucose occurred in three steps. First, a self-assembled monolayer of 11-mercaptoundecanoic acid (MUA) was formed at the porous surface of the reactor. Second, enzyme glucose oxidase (GOx) was covalently immobilized at MUA-layer using N-ethyl-N-(3-dimethylaminopropyl) carboimide and N-hydroxysuccinimide chemistry. Finally, a decrease of oxygen concentration (directly proportional to the concentration of glucose) during enzymatic reaction was amperometrically measured on the tubular detector under flow injection conditions. The following parameters of glucose determination were optimized with respect to amperometric response: composition of the mobile phase, its concentration, the potential of detection and the

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP206%2F11%2F1638" target="_blank" >GAP206/11/1638: Nové elektrochemické senzory a detekční techniky pro analýzu struktury a interakcí nukleových kyselin</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytica Chimica Acta

ISSN

0003-2670

e-ISSN

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Svazek periodika

778

Číslo periodika v rámci svazku

MAY 2013

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

24-30

Kód UT WoS článku

000318959400004

EID výsledku v databázi Scopus

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