Covalent immobilization of redox protein within the mesopores of transparent conducting electrodes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F14%3A00421301" target="_blank" >RIV/61388955:_____/14:00421301 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.electacta.2013.10.136" target="_blank" >http://dx.doi.org/10.1016/j.electacta.2013.10.136</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.electacta.2013.10.136" target="_blank" >10.1016/j.electacta.2013.10.136</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Covalent immobilization of redox protein within the mesopores of transparent conducting electrodes

Popis výsledku v původním jazyce

Redox protein cytochrome c was immobilized at high electrochemically accessible loading on mesoporous films of antimony doped tin oxide (ATO) exhibiting high conductivity, transparency and a large surface area. The grafting was achieved by covalent attachment of the protein to the electrode surface. Alternatively, cytochrome c was effectively adsorbed on the ATO electrode surface due to strong electrostatic interaction between the positively charged cytochrome c and the negatively charged ATO surface. The amount of electrochemically addressable cytochrome c is proportional to the specific surface area, reaching up to 440pmol/cm2 and 600pmol/cm2 for covalently attached and adsorbed protein, respectively, for the 370nm thick films. The covalently attached protein exhibits substantially higher stability towards leaching than the adsorbed one. The combination of transparent conducting porous electrode matrix with the electroactive proteins is promising for the development of efficient bio-

Název v anglickém jazyce

Covalent immobilization of redox protein within the mesopores of transparent conducting electrodes

Popis výsledku anglicky

Redox protein cytochrome c was immobilized at high electrochemically accessible loading on mesoporous films of antimony doped tin oxide (ATO) exhibiting high conductivity, transparency and a large surface area. The grafting was achieved by covalent attachment of the protein to the electrode surface. Alternatively, cytochrome c was effectively adsorbed on the ATO electrode surface due to strong electrostatic interaction between the positively charged cytochrome c and the negatively charged ATO surface. The amount of electrochemically addressable cytochrome c is proportional to the specific surface area, reaching up to 440pmol/cm2 and 600pmol/cm2 for covalently attached and adsorbed protein, respectively, for the 370nm thick films. The covalently attached protein exhibits substantially higher stability towards leaching than the adsorbed one. The combination of transparent conducting porous electrode matrix with the electroactive proteins is promising for the development of efficient bio-

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA104%2F08%2F0435" target="_blank" >GA104/08/0435: "Inteligentně strukturované" mesoporézní vrstvy TiO2 s antibakteriálními a řízeně proměnnými smáčecími vlastnostmi</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrochimica acta

ISSN

0013-4686

e-ISSN

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Svazek periodika

116

Číslo periodika v rámci svazku

JAN 2014

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

1-8

Kód UT WoS článku

000331494400001

EID výsledku v databázi Scopus

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