CYTOTOXICITY OF NANOMATERIALS APPLICABLE IN RESTORATION AND CONSERVATION

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F19%3A00522950" target="_blank" >RIV/61388955:_____/19:00522950 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378041:_____/19:00522950 RIV/61989100:27200/19:10242336

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

CYTOTOXICITY OF NANOMATERIALS APPLICABLE IN RESTORATION AND CONSERVATION

Popis výsledku v původním jazyce

Nanotechnologies represent new promising approach for conservation and preservation of cultural heritage. Nanomaterials (NMs) have been shown to improve efficacy, durability and quality of the restoration due to their unique properties that can be achieved and tuned by controlling particle physico-chemical characteristics. The same characteristics, however, affect the way how NMs interact with biological systems and may potentially underlie their enhanced or unexpected toxicity. In this pilot study, we compared the toxic potential of representatives of three of the most common oxide materials applicable in restoration: TiO2 (standard and purified P25, a mixture of prevailing anatase with rutile crystalline modifications), SiO2 (bare A200, and R805, R9200 as coated forms of A200), and ZnO. Using two in vitro cytotoxicity assays, WST-1 and LDH, evaluating metabolic activity and cell membrane integrity, respectively, we preliminary ranked the tested substances according to their cytotoxic potential, which may be used for their prioritization for further testing and applications. After 24h exposure, a dose-dependent decrease in cell viability was only detected in ZnO NPs and uncoated silica (A200). Hydrophobic coated silicas (R805 and R9200) and TiO2 NPs (purified and unpurified P25) did not exhibit cytotoxic effects up to the highest tested concentration of 250 mu g/mL. Toxicological data related to the physico-chemical characteristics will be applicable in developing both more efficient and safer nano-based products for restoration and conservation.

Název v anglickém jazyce

CYTOTOXICITY OF NANOMATERIALS APPLICABLE IN RESTORATION AND CONSERVATION

Popis výsledku anglicky

Nanotechnologies represent new promising approach for conservation and preservation of cultural heritage. Nanomaterials (NMs) have been shown to improve efficacy, durability and quality of the restoration due to their unique properties that can be achieved and tuned by controlling particle physico-chemical characteristics. The same characteristics, however, affect the way how NMs interact with biological systems and may potentially underlie their enhanced or unexpected toxicity. In this pilot study, we compared the toxic potential of representatives of three of the most common oxide materials applicable in restoration: TiO2 (standard and purified P25, a mixture of prevailing anatase with rutile crystalline modifications), SiO2 (bare A200, and R805, R9200 as coated forms of A200), and ZnO. Using two in vitro cytotoxicity assays, WST-1 and LDH, evaluating metabolic activity and cell membrane integrity, respectively, we preliminary ranked the tested substances according to their cytotoxic potential, which may be used for their prioritization for further testing and applications. After 24h exposure, a dose-dependent decrease in cell viability was only detected in ZnO NPs and uncoated silica (A200). Hydrophobic coated silicas (R805 and R9200) and TiO2 NPs (purified and unpurified P25) did not exhibit cytotoxic effects up to the highest tested concentration of 250 mu g/mL. Toxicological data related to the physico-chemical characteristics will be applicable in developing both more efficient and safer nano-based products for restoration and conservation.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10403 - Physical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application

ISBN

978-808729489-5

ISSN

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e-ISSN

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Počet stran výsledku

6

Strana od-do

548-553

Název nakladatele

Tanger Ltd.

Místo vydání

Ostrava

Místo konání akce

Brno

Datum konání akce

17. 10. 2018

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

000513131900095