Cytotoxicity Induced by Nanoparticles - the Standardisation of Methods within the QualityNano EP7 Network

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378041%3A_____%2F15%3A00459251" target="_blank" >RIV/68378041:_____/15:00459251 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Cytotoxicity Induced by Nanoparticles - the Standardisation of Methods within the QualityNano EP7 Network

Popis výsledku v původním jazyce

The uniqueness and diversity of nanomaterials (NMs) raise questions regarding their safety, as well as about feasible ways to assess their potential toxicity. Despite intensive nanotoxicological research, it is impossible to keep pace with the rate of development and production of new NMs. Modifications of NMs’ size, shape, and numerous other characteristics are performed to reach desirable industrial properties. To be able to alter, or rather minimize, the toxicity of NMs in the same way, we have to understand which NMs properties govern particular toxic effects. Computational modeling relating the physico-chemical properties of NMs with their toxic effects holds great promise in this area; however, the lack of high-quality comparable experimental data of properly characterized NMs hampers the development of reliable models. In the present study, we evaluated the cytotoxic potential of a set of TiO2 nanomaterials differing in basic characteristics (crystal structure, size, shape) using three in vitro assays (MTS, WST-1, LDH) and three human cell lines (alveolar A549 cells and bronchial BEAS-2B, representing in vitro models for studying inhalation toxicity, and PMA-differentiated THP-1 as a model of macrophages). TiO2 is one of the most frequently used nanomaterials. Although inert in the bulk form, some TiO2 nanoforms have been reported to elicit a toxic response. Our study compares the cytotoxicity results of 14 nano TiO2 variants in relation to their physico-chemical properties. Moreover, the sensitivity of the employed testing methods and cell lines was compared. The main purpose of the study was to generate high-quality data for the computational modeling of cytotoxicity (nano-QSAR).

Název v anglickém jazyce

Cytotoxicity Induced by Nanoparticles - the Standardisation of Methods within the QualityNano EP7 Network

Popis výsledku anglicky

The uniqueness and diversity of nanomaterials (NMs) raise questions regarding their safety, as well as about feasible ways to assess their potential toxicity. Despite intensive nanotoxicological research, it is impossible to keep pace with the rate of development and production of new NMs. Modifications of NMs’ size, shape, and numerous other characteristics are performed to reach desirable industrial properties. To be able to alter, or rather minimize, the toxicity of NMs in the same way, we have to understand which NMs properties govern particular toxic effects. Computational modeling relating the physico-chemical properties of NMs with their toxic effects holds great promise in this area; however, the lack of high-quality comparable experimental data of properly characterized NMs hampers the development of reliable models. In the present study, we evaluated the cytotoxic potential of a set of TiO2 nanomaterials differing in basic characteristics (crystal structure, size, shape) using three in vitro assays (MTS, WST-1, LDH) and three human cell lines (alveolar A549 cells and bronchial BEAS-2B, representing in vitro models for studying inhalation toxicity, and PMA-differentiated THP-1 as a model of macrophages). TiO2 is one of the most frequently used nanomaterials. Although inert in the bulk form, some TiO2 nanoforms have been reported to elicit a toxic response. Our study compares the cytotoxicity results of 14 nano TiO2 variants in relation to their physico-chemical properties. Moreover, the sensitivity of the employed testing methods and cell lines was compared. The main purpose of the study was to generate high-quality data for the computational modeling of cytotoxicity (nano-QSAR).

Druh

C - Kapitola v odborné knize

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/LO1508" target="_blank" >LO1508: Genomika a proteomika při studiu mechanismů biologických účinků vyráběných nanočástic</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

From Functionalized Nanostructures Towards Engineered Macrostructures

ISBN

978-80-88113-20-1

Počet stran výsledku

11

Strana od-do

24-34

Počet stran knihy

176

Název nakladatele

Epika

Místo vydání

Jindřichův Hradec

Kód UT WoS kapitoly

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