New fluorescent-labelled nanoparticles: synthesis, characterization and interactions with cysteine and homocysteine to evaluate their stability in aqueous solution

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F20%3A00536189" target="_blank" >RIV/61388955:_____/20:00536189 - isvavai.cz</a>

Výsledek na webu

<a href="http://hdl.handle.net/11104/0313999" target="_blank" >http://hdl.handle.net/11104/0313999</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13204-019-01241-9" target="_blank" >10.1007/s13204-019-01241-9</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New fluorescent-labelled nanoparticles: synthesis, characterization and interactions with cysteine and homocysteine to evaluate their stability in aqueous solution

Popis výsledku v původním jazyce

Fluorescent-labelled nanoparticles conjugate the SPR of nanomaterials as well as the fluorescence properties of the capping dye. In this work, we report a study on the synthesis of fluorescent l-tyrosine (l-Tyr) and fluorescein (Fluo)-capped silver nanoparticles (AgNPs) carried out by a fine-tuning of the analytical concentration of the reagents. The AgNPs have been characterized by TEM, UV-Vis, ATR-FTIR, and photoluminescence (PL) spectroscopy and DLS. The adsorption of cysteine and homocysteine on the surface of the nanoparticles has been studied to evaluate their overall evolution in solution and their possible interactions with more complex systems, such as proteins. Opposed to homocysteine, cysteine induces aggregation either of tyrosine- and fluorescein-capped nanoparticles, which are therefore promising systems for the discrimination of biothiols. Furthermore, tyrosine-capped AgNPs, in spite of the better coordinating characteristics of this amino acid with respect to fluorescein, show aggregation abilities with cysteine greater than the fluorescein-capped ones that are unexpectedly more stable and thus less prone to aggregation phenomena.

Název v anglickém jazyce

New fluorescent-labelled nanoparticles: synthesis, characterization and interactions with cysteine and homocysteine to evaluate their stability in aqueous solution

Popis výsledku anglicky

Fluorescent-labelled nanoparticles conjugate the SPR of nanomaterials as well as the fluorescence properties of the capping dye. In this work, we report a study on the synthesis of fluorescent l-tyrosine (l-Tyr) and fluorescein (Fluo)-capped silver nanoparticles (AgNPs) carried out by a fine-tuning of the analytical concentration of the reagents. The AgNPs have been characterized by TEM, UV-Vis, ATR-FTIR, and photoluminescence (PL) spectroscopy and DLS. The adsorption of cysteine and homocysteine on the surface of the nanoparticles has been studied to evaluate their overall evolution in solution and their possible interactions with more complex systems, such as proteins. Opposed to homocysteine, cysteine induces aggregation either of tyrosine- and fluorescein-capped nanoparticles, which are therefore promising systems for the discrimination of biothiols. Furthermore, tyrosine-capped AgNPs, in spite of the better coordinating characteristics of this amino acid with respect to fluorescein, show aggregation abilities with cysteine greater than the fluorescein-capped ones that are unexpectedly more stable and thus less prone to aggregation phenomena.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Nanoscience

ISSN

2190-5509

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

SA - Království Saúdská Arábie

Počet stran výsledku

16

Strana od-do

1157-1172

Kód UT WoS článku

000520600600014

EID výsledku v databázi Scopus

2-s2.0-85077522769