Phospholipid modified glassy carbon electrode for determination of chili peppers pungency by ex-situ extraction voltammetry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F20%3A00537142" target="_blank" >RIV/61388955:_____/20:00537142 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61989592:15310/20:73603866

Výsledek na webu

<a href="http://hdl.handle.net/11104/0314891" target="_blank" >http://hdl.handle.net/11104/0314891</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jelechem.2019.113790" target="_blank" >10.1016/j.jelechem.2019.113790</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Phospholipid modified glassy carbon electrode for determination of chili peppers pungency by ex-situ extraction voltammetry

Popis výsledku v původním jazyce

Objective determination of chili peppers pungency, for which capsaicinoids (CPDs), predominantly capsaicin (CA) and dihydrocapsaicin (DHC) are responsible, belongs to frequently solved tasks in food and medicinal analysis. In this manuscript, application of ex situ extractive stripping voltammetry (ExSV) utilizing a phospholipid modified glassy carbon electrode is described.nThe obtained analytical parameters, e.g., linear dynamic range from 3 to 60 mg L-1, limit of detection 2.1 mg L-1, proved to be fully comparable with those achieved using the direct voltammetric method on a polished glassy carbon electrode and reference HPLC/UV. Although the sensitivity of ExSV was slightly lower compared to the direct method, ExSV provided better selectivity to CPDs due to their lipophilic interactions with the phospholipid layer. Hydrophilic components of the chili pepper samples were not extracted into the phospholipid layer and therefore did not interfere. The developed method was tested on the determination of CPDs in different chili peppers: Foxta, Madras Chilli, Habanero, Carolina Reaper, Bird's eye, Bhut Jolokia, Naga Jolokia extract, and Habanero extract.nFurthermore, the electrochemical reactions of CPDs in acidic aqueous-methanolic media were characterized using an electrochemical cell hyphenated with mass spectrometer and the main oxidation products were identified.

Název v anglickém jazyce

Phospholipid modified glassy carbon electrode for determination of chili peppers pungency by ex-situ extraction voltammetry

Popis výsledku anglicky

Objective determination of chili peppers pungency, for which capsaicinoids (CPDs), predominantly capsaicin (CA) and dihydrocapsaicin (DHC) are responsible, belongs to frequently solved tasks in food and medicinal analysis. In this manuscript, application of ex situ extractive stripping voltammetry (ExSV) utilizing a phospholipid modified glassy carbon electrode is described.nThe obtained analytical parameters, e.g., linear dynamic range from 3 to 60 mg L-1, limit of detection 2.1 mg L-1, proved to be fully comparable with those achieved using the direct voltammetric method on a polished glassy carbon electrode and reference HPLC/UV. Although the sensitivity of ExSV was slightly lower compared to the direct method, ExSV provided better selectivity to CPDs due to their lipophilic interactions with the phospholipid layer. Hydrophilic components of the chili pepper samples were not extracted into the phospholipid layer and therefore did not interfere. The developed method was tested on the determination of CPDs in different chili peppers: Foxta, Madras Chilli, Habanero, Carolina Reaper, Bird's eye, Bhut Jolokia, Naga Jolokia extract, and Habanero extract.nFurthermore, the electrochemical reactions of CPDs in acidic aqueous-methanolic media were characterized using an electrochemical cell hyphenated with mass spectrometer and the main oxidation products were identified.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/GA17-05387S" target="_blank" >GA17-05387S: Nové přístupy pro sledování přenosu zvolených xenobiotik přes biomimetické membrány</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Electroanalytical Chemistry

ISSN

1572-6657

e-ISSN

—

Svazek periodika

858

Číslo periodika v rámci svazku

FEB 2020

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

9

Strana od-do

113790

Kód UT WoS článku

000515205600034

EID výsledku v databázi Scopus

2-s2.0-85077722918