Expression and Localization of AβPP in SH-SY5Y Cells Depends on Differentiation State

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F21%3A00544239" target="_blank" >RIV/61388955:_____/21:00544239 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11120/21:43921594 RIV/00216208:11310/21:10428066

Výsledek na webu

<a href="http://hdl.handle.net/11104/0321269" target="_blank" >http://hdl.handle.net/11104/0321269</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3233/JAD-201409" target="_blank" >10.3233/JAD-201409</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Expression and Localization of AβPP in SH-SY5Y Cells Depends on Differentiation State

Popis výsledku v původním jazyce

Neuroblastoma cell line SH-SY5Y, due to its capacity to differentiate into neurons, easy handling, and low cost, is a common experimental model to study molecular events leading to Alzheimer's disease (AD). However, it is prevalently used in its undifferentiated state, which does not resemble neurons affected by the disease. Here, we show that the expression and localization of amyloid-β protein precursor (AβPP), one of the key molecules involved in AD pathogenesis, is dramatically altered in SH-SY5Y cells fully differentiated by combined treatment with retinoic acid and BDNF. We show that insufficient differentiation of SH-SY5Y cells results in AβPP mislocalization.n

Název v anglickém jazyce

Expression and Localization of AβPP in SH-SY5Y Cells Depends on Differentiation State

Popis výsledku anglicky

Neuroblastoma cell line SH-SY5Y, due to its capacity to differentiate into neurons, easy handling, and low cost, is a common experimental model to study molecular events leading to Alzheimer's disease (AD). However, it is prevalently used in its undifferentiated state, which does not resemble neurons affected by the disease. Here, we show that the expression and localization of amyloid-β protein precursor (AβPP), one of the key molecules involved in AD pathogenesis, is dramatically altered in SH-SY5Y cells fully differentiated by combined treatment with retinoic acid and BDNF. We show that insufficient differentiation of SH-SY5Y cells results in AβPP mislocalization.n

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

<a href="/cs/project/GJ19-08304Y" target="_blank" >GJ19-08304Y: Vliv mTORC1 signalizace na vnitrobuněčné procesování a lokalizaci amyloidního prekurzoru a jeho derivátů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Alzheimer's Disease

ISSN

1387-2877

e-ISSN

1875-8908

Svazek periodika

82

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

485-491

Kód UT WoS článku

000677582000003

EID výsledku v databázi Scopus

2-s2.0-85111397319