Antibacterial Nanoparticles with Natural Photosensitizers Extracted from Spinach Leaves

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F22%3A00551259" target="_blank" >RIV/61388955:_____/22:00551259 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/22:10445190

Výsledek na webu

<a href="http://hdl.handle.net/11104/0326699http://hdl.handle.net/11104/0326699" target="_blank" >http://hdl.handle.net/11104/0326699http://hdl.handle.net/11104/0326699</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acsomega.1c06229" target="_blank" >10.1021/acsomega.1c06229</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Antibacterial Nanoparticles with Natural Photosensitizers Extracted from Spinach Leaves

Popis výsledku v původním jazyce

We prepared antibacterial polystyrene nanoparticles (NPs) with natural photosensitizers from chlorophyll (Chl) extract via a simple nanoprecipitation method using the same solvent for dissolution of the polystyrene matrix and extraction of Chls from spinach leaves. A high photo-oxidation and antibacterial effect was demonstrated on Escherichia coli and was based on the photo generation of singlet oxygen O-2((1)Delta(g)), which was directly monitored by NIR luminescence measurements and indirectly verified using a chemical trap. The photoactivity of NPs was triggered by visible light, with enhanced red absorption by Chls. To reduce the quenching effect of carotenoids (beta-carotene, lutein, etc.) in the Chl extract, diluted and/or preirradiated samples, in which the photo-oxidized carotenoids lose their quenching effect, were used for preparation of the NPs. For enhanced photo-oxidation and antibacterial effects, a sulfonated polystyrene matrix was used for preparation of a stable dispersion of sulfonated NPs, with the quenching effect of carotenoids being suppressed.

Název v anglickém jazyce

Antibacterial Nanoparticles with Natural Photosensitizers Extracted from Spinach Leaves

Popis výsledku anglicky

We prepared antibacterial polystyrene nanoparticles (NPs) with natural photosensitizers from chlorophyll (Chl) extract via a simple nanoprecipitation method using the same solvent for dissolution of the polystyrene matrix and extraction of Chls from spinach leaves. A high photo-oxidation and antibacterial effect was demonstrated on Escherichia coli and was based on the photo generation of singlet oxygen O-2((1)Delta(g)), which was directly monitored by NIR luminescence measurements and indirectly verified using a chemical trap. The photoactivity of NPs was triggered by visible light, with enhanced red absorption by Chls. To reduce the quenching effect of carotenoids (beta-carotene, lutein, etc.) in the Chl extract, diluted and/or preirradiated samples, in which the photo-oxidized carotenoids lose their quenching effect, were used for preparation of the NPs. For enhanced photo-oxidation and antibacterial effects, a sulfonated polystyrene matrix was used for preparation of a stable dispersion of sulfonated NPs, with the quenching effect of carotenoids being suppressed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACS Omega

ISSN

2470-1343

e-ISSN

2470-1343

Svazek periodika

7

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

1505-1513

Kód UT WoS článku

000734279500001

EID výsledku v databázi Scopus

2-s2.0-85122036780