Cytoplasmic fluidity and the cold life: proteome stability is decoupled from viability in psychrophiles

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F25%3A00642143" target="_blank" >RIV/61388955:_____/25:00642143 - isvavai.cz</a>

Výsledek na webu

<a href="https://hdl.handle.net/11104/0372063" target="_blank" >https://hdl.handle.net/11104/0372063</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41467-025-65270-5" target="_blank" >10.1038/s41467-025-65270-5</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Cytoplasmic fluidity and the cold life: proteome stability is decoupled from viability in psychrophiles

Popis výsledku v původním jazyce

Protein diffusion, critical for cellular metabolism, occurs in the highly crowded cytoplasm. Understanding how this dynamics changes when organisms are adapted to different thermal niches is a fundamental challenge in microbiology and biophysics. In Escherichia coli, protein diffusion undergoes a pronounced slowdown at temperatures near cellular death, coinciding with the early stages of unfolding. To determine whether this phenomenon is universal, we investigated psychrophilic and hyperthermophilic bacteria. In both species, a marked diffusion slowdown takes place at the onset of proteome melting. However, while the dynamic arrest is associated with the thermal death point for the hyperthermophilic proteome, the psychrophilic proteome maintains substantial mobility well beyond the cellular inactivation. The decoupling between metabolic viability and proteome dynamics and stability suggests that the functional processes of psychrophilic bacteria are temperature sensitive. This finding echoes the behavior of psychrophilic enzymes, manifesting a large temperature gap between optimal activity and unfolding. Protein diffusion is optimized to maintain functional fluidity at the organism’s working conditions, but its temperature dependence is controlled by the proteome folded state. Our findings redefine the relationship between cytoplasmic dynamics, proteome stability, and bacterial survival in cold environments.

Název v anglickém jazyce

Cytoplasmic fluidity and the cold life: proteome stability is decoupled from viability in psychrophiles

Popis výsledku anglicky

Protein diffusion, critical for cellular metabolism, occurs in the highly crowded cytoplasm. Understanding how this dynamics changes when organisms are adapted to different thermal niches is a fundamental challenge in microbiology and biophysics. In Escherichia coli, protein diffusion undergoes a pronounced slowdown at temperatures near cellular death, coinciding with the early stages of unfolding. To determine whether this phenomenon is universal, we investigated psychrophilic and hyperthermophilic bacteria. In both species, a marked diffusion slowdown takes place at the onset of proteome melting. However, while the dynamic arrest is associated with the thermal death point for the hyperthermophilic proteome, the psychrophilic proteome maintains substantial mobility well beyond the cellular inactivation. The decoupling between metabolic viability and proteome dynamics and stability suggests that the functional processes of psychrophilic bacteria are temperature sensitive. This finding echoes the behavior of psychrophilic enzymes, manifesting a large temperature gap between optimal activity and unfolding. Protein diffusion is optimized to maintain functional fluidity at the organism’s working conditions, but its temperature dependence is controlled by the proteome folded state. Our findings redefine the relationship between cytoplasmic dynamics, proteome stability, and bacterial survival in cold environments.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Nature Communications

ISSN

2041-1723

e-ISSN

2041-1723

Svazek periodika

16

Číslo periodika v rámci svazku

NOV 2025

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

13

Strana od-do

10345

Kód UT WoS článku

001624438000016

EID výsledku v databázi Scopus

2-s2.0-105022738312