The library of simulated STED-FCS diffusion law plots

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F26%3A00647048" target="_blank" >RIV/61388955:_____/26:00647048 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The library of simulated STED-FCS diffusion law plots

Popis výsledku v původním jazyce

Formation of functional nanoscopic domains is an inherent property of plasma membranes. Stimulated emission depletion combined with fluorescence correlation spectroscopy (STED-FCS) has been used to identify such domains, however, the information obtained by STED-FCS has been limited to presence of such domains while crucial parameters have not been accessible, such as size, the fraction of occupied membrane surface, in-membrane lipid diffusion inside and outside the nanodomains as well as their self-diffusion. Here, based on a revision of the ‘diffusion law’, we present an approach to retrieve these five parameters from STED-FCS data. We verify that approach on ganglioside nanodomains in giant unilamellar vesicles (GUVs), validating the Saffman-Delbrück assumption for domain diffusion.

Název v anglickém jazyce

The library of simulated STED-FCS diffusion law plots

Popis výsledku anglicky

Formation of functional nanoscopic domains is an inherent property of plasma membranes. Stimulated emission depletion combined with fluorescence correlation spectroscopy (STED-FCS) has been used to identify such domains, however, the information obtained by STED-FCS has been limited to presence of such domains while crucial parameters have not been accessible, such as size, the fraction of occupied membrane surface, in-membrane lipid diffusion inside and outside the nanodomains as well as their self-diffusion. Here, based on a revision of the ‘diffusion law’, we present an approach to retrieve these five parameters from STED-FCS data. We verify that approach on ganglioside nanodomains in giant unilamellar vesicles (GUVs), validating the Saffman-Delbrück assumption for domain diffusion.

Druh

X - Nezařazeno

CEP obor

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OECD FORD obor

10610 - Biophysics

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2026

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů