2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F11%3A00360024" target="_blank" >RIV/61388963:_____/11:00360024 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.4161/epi.6.6.16215" target="_blank" >http://dx.doi.org/10.4161/epi.6.6.16215</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.4161/epi.6.6.16215" target="_blank" >10.4161/epi.6.6.16215</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential
Popis výsledku v původním jazyce
A comparative study of hypomethylating activities of a series of 5-azacytidine nucleosides: 5-azacytidine, 2´-deoxy-5-azacytidine and its alpha-anomer, 5,6-dihydro-5-azacytidine, 2´-deoxy-5,6-dihydro-5-azacytidine and its ?-anomer, and of a 2-pyrimidoneribonucleoside (zebularine) was conducted. Methylation-specific PCR was employed to detect the efficiency of individual agents on cyclin-dependent kinase inhibitor 2B and thrombospondin-1 hypermethylated gene loci. Overall changes in DNA methylation level were quantified by direct estimation of 5-methyl-2´-deoxycytidine 5´-monophosphate by HPLC using digested genomic DNA. Flow cytometric analysis of cell cycle progression and apoptotic markers was used to determine cytotoxicity of the compounds. mRNA expression was measured using qRT-PCR. 2´-Deoxy-5,6-dihydro-5-azacytidine was found to be less cytotoxic and more stable than 2´-deoxy-5-azacytidine at the doses that induce comparable DNA hypomethylation and gene reactivation.
Název v anglickém jazyce
2'-deoxy-5,6-dihydro-5-azacytidine-a less toxic alternative of 2'-deoxy-5-azacytidine. A comparative study of hypomethylating potential
Popis výsledku anglicky
A comparative study of hypomethylating activities of a series of 5-azacytidine nucleosides: 5-azacytidine, 2´-deoxy-5-azacytidine and its alpha-anomer, 5,6-dihydro-5-azacytidine, 2´-deoxy-5,6-dihydro-5-azacytidine and its ?-anomer, and of a 2-pyrimidoneribonucleoside (zebularine) was conducted. Methylation-specific PCR was employed to detect the efficiency of individual agents on cyclin-dependent kinase inhibitor 2B and thrombospondin-1 hypermethylated gene loci. Overall changes in DNA methylation level were quantified by direct estimation of 5-methyl-2´-deoxycytidine 5´-monophosphate by HPLC using digested genomic DNA. Flow cytometric analysis of cell cycle progression and apoptotic markers was used to determine cytotoxicity of the compounds. mRNA expression was measured using qRT-PCR. 2´-Deoxy-5,6-dihydro-5-azacytidine was found to be less cytotoxic and more stable than 2´-deoxy-5-azacytidine at the doses that induce comparable DNA hypomethylation and gene reactivation.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/1M0508" target="_blank" >1M0508: Nová antivirotika a antineoplastika</a><br>
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Epigenetics
ISSN
1559-2294
e-ISSN
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Svazek periodika
6
Číslo periodika v rámci svazku
6
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
8
Strana od-do
769-776
Kód UT WoS článku
000291148100013
EID výsledku v databázi Scopus
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