Specific features of Zn2+, Co2+ and Ni2+ ion binding to DNA in alkaline solutions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00392688" target="_blank" >RIV/61388963:_____/13:00392688 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2013.01.001" target="_blank" >http://dx.doi.org/10.1016/j.ijbiomac.2013.01.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2013.01.001" target="_blank" >10.1016/j.ijbiomac.2013.01.001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Specific features of Zn2+, Co2+ and Ni2+ ion binding to DNA in alkaline solutions

Popis výsledku v původním jazyce

Dependence of DNA metallization degree during B-DNA transition into the metallized (m) form on DNA concentration has been studied by visible and differential UV-spectroscopy in the presence of Zn2+, Co2+ and Ni2+ ions in tetraborate buffer (pH 8.5) withand without ethidium bromide. Constants of Mt(2+) binding to double stranded DNA were calculated. The obtained binding constants corresponded to the formation of inter-strand metal bridges stabilizing m-form. Thermodynamic origin of higher efficiency ofZn2+ ions in DNA metallization compared to Co2+ and Ni2+ was revealed. Increase of the DNA helix-coil transition temperature by up to 10 degrees C upon formation of m-form in the presence of Zn2+ ions was observed and rationalized. Furthermore, a strongcooperative decrease (up to 30 degrees C) of the temperature of B m transition induced by heating in the presence of Zn2+ was found and its nature was explained.

Název v anglickém jazyce

Specific features of Zn2+, Co2+ and Ni2+ ion binding to DNA in alkaline solutions

Popis výsledku anglicky

Dependence of DNA metallization degree during B-DNA transition into the metallized (m) form on DNA concentration has been studied by visible and differential UV-spectroscopy in the presence of Zn2+, Co2+ and Ni2+ ions in tetraborate buffer (pH 8.5) withand without ethidium bromide. Constants of Mt(2+) binding to double stranded DNA were calculated. The obtained binding constants corresponded to the formation of inter-strand metal bridges stabilizing m-form. Thermodynamic origin of higher efficiency ofZn2+ ions in DNA metallization compared to Co2+ and Ni2+ was revealed. Increase of the DNA helix-coil transition temperature by up to 10 degrees C upon formation of m-form in the presence of Zn2+ ions was observed and rationalized. Furthermore, a strongcooperative decrease (up to 30 degrees C) of the temperature of B m transition induced by heating in the presence of Zn2+ was found and its nature was explained.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP208%2F10%2F0559" target="_blank" >GAP208/10/0559: Teoretické a spektroskopické studie agregátů nukleových kyselin</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

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Svazek periodika

55

Číslo periodika v rámci svazku

Apr

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

137-141

Kód UT WoS článku

000316778400020

EID výsledku v databázi Scopus

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