Effect of Zn2+ and temperature on the conformational equilibrium of single-stranded polyA in neutral solutions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00420878" target="_blank" >RIV/61388963:_____/13:00420878 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2013.08.010" target="_blank" >http://dx.doi.org/10.1016/j.ijbiomac.2013.08.010</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijbiomac.2013.08.010" target="_blank" >10.1016/j.ijbiomac.2013.08.010</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of Zn2+ and temperature on the conformational equilibrium of single-stranded polyA in neutral solutions

Popis výsledku v původním jazyce

Effect of Zn2+ ions on the conformation of polyA in cacodilic buffer at pH 7 was investigated by differential UV spectroscopy (DUV) and by thermal denaturation. The shapes of the DUV spectra and melting curves suggest a transition of polyA into a more ordered "metallized", possibly double-helical conformation at Zn2+ concentrations above 3 x 10(-5) M. A phase diagram of polyA complexes with Zn2+ was constructed for the temperature range from 20 degrees C to 95 degrees C and Zn2+ concentrations between 10(-5) M and 5 x 10(-4) M. It was found that the transition of a single strand into the "metallized" form is possible only if the length of the disordered single-stranded region becomes larger than a certain critical value, ranging between 98% and 78% asthe metal concentration increases from 3 x 10(-5) to 5 x 10(-4) M.

Název v anglickém jazyce

Effect of Zn2+ and temperature on the conformational equilibrium of single-stranded polyA in neutral solutions

Popis výsledku anglicky

Effect of Zn2+ ions on the conformation of polyA in cacodilic buffer at pH 7 was investigated by differential UV spectroscopy (DUV) and by thermal denaturation. The shapes of the DUV spectra and melting curves suggest a transition of polyA into a more ordered "metallized", possibly double-helical conformation at Zn2+ concentrations above 3 x 10(-5) M. A phase diagram of polyA complexes with Zn2+ was constructed for the temperature range from 20 degrees C to 95 degrees C and Zn2+ concentrations between 10(-5) M and 5 x 10(-4) M. It was found that the transition of a single strand into the "metallized" form is possible only if the length of the disordered single-stranded region becomes larger than a certain critical value, ranging between 98% and 78% asthe metal concentration increases from 3 x 10(-5) to 5 x 10(-4) M.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP208%2F10%2F0559" target="_blank" >GAP208/10/0559: Teoretické a spektroskopické studie agregátů nukleových kyselin</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

International Journal of Biological Macromolecules

ISSN

0141-8130

e-ISSN

—

Svazek periodika

61

Číslo periodika v rámci svazku

Oct

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

448-452

Kód UT WoS článku

000326660700065

EID výsledku v databázi Scopus

—