Interaction of Zn2+ Ions with Single-Stranded PolyU and PolyC in Neutral Solutions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F15%3A00443982" target="_blank" >RIV/61388963:_____/15:00443982 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1021/acs.jpcb.5b00558" target="_blank" >http://dx.doi.org/10.1021/acs.jpcb.5b00558</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.jpcb.5b00558" target="_blank" >10.1021/acs.jpcb.5b00558</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of Zn2+ Ions with Single-Stranded PolyU and PolyC in Neutral Solutions

Popis výsledku v původním jazyce

Effect of Zn2+ ions on the conformation of single-stranded polynucleotides polyU and polyC in a wide temperature range at pH 7 was studied by differential UV spectroscopy and by thermal denaturation. The atoms coordinating Zn2+ ions were determined (O4 and N3 in polyU and N3 in polyC). A three-dimensional phase diagram and its two-dimensional components were constructed for a polyC-Zn2+ system. The phase diagram revealed a region in which ordered single-stranded structures, stabilized by Zn2+-mediated cross-links involving N3 atom of cytosine, are formed. The phase diagram also demonstrated that the behavior of the polyC-Zn2+ system is similar to the effect of retrograde condensation observed in some binary solutions of simple liquids. A dependence ofZn2+-polyC binding constant on the metal ion concentration was obtained. The reason why zinc-induced transition of the sequences with adenine-uracil (AU) base pairs from A-form geometry to a metallized m-form requires higher pH compared t

Název v anglickém jazyce

Interaction of Zn2+ Ions with Single-Stranded PolyU and PolyC in Neutral Solutions

Popis výsledku anglicky

Effect of Zn2+ ions on the conformation of single-stranded polynucleotides polyU and polyC in a wide temperature range at pH 7 was studied by differential UV spectroscopy and by thermal denaturation. The atoms coordinating Zn2+ ions were determined (O4 and N3 in polyU and N3 in polyC). A three-dimensional phase diagram and its two-dimensional components were constructed for a polyC-Zn2+ system. The phase diagram revealed a region in which ordered single-stranded structures, stabilized by Zn2+-mediated cross-links involving N3 atom of cytosine, are formed. The phase diagram also demonstrated that the behavior of the polyC-Zn2+ system is similar to the effect of retrograde condensation observed in some binary solutions of simple liquids. A dependence ofZn2+-polyC binding constant on the metal ion concentration was obtained. The reason why zinc-induced transition of the sequences with adenine-uracil (AU) base pairs from A-form geometry to a metallized m-form requires higher pH compared t

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Physical Chemistry B

ISSN

1520-6106

e-ISSN

—

Svazek periodika

119

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

4409-4416

Kód UT WoS článku

000351971100007

EID výsledku v databázi Scopus

2-s2.0-84925935687