Determination of acidity constants and ionic mobilities of polyprotic peptide hormones by CZE

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00421961" target="_blank" >RIV/61388963:_____/13:00421961 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/elps.201300119" target="_blank" >http://dx.doi.org/10.1002/elps.201300119</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201300119" target="_blank" >10.1002/elps.201300119</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Determination of acidity constants and ionic mobilities of polyprotic peptide hormones by CZE

Popis výsledku v původním jazyce

CZE has been applied to determination of thermodynamic acidity constants (pK(a)) of ionogenic groups and actual ionic mobilities of polyprotic peptides-synthetic human and salmon gonadotropin-releasing hormones and their derivatives and fragments. First,the mixed acidity constants, pK(a,i)(mix), of ionogenic groups, and actual ionic mobilities, m(i), of gonadotropin-releasing hormone peptides were determined by nonlinear regression analysis of pH dependence of their effective electrophoretic mobilities. The effective mobilities were measured by CZE in a series of BGEs within a broad pH range (1.80-12.10), at constant ionic strength (25 mM) and reference temperature (25 degrees C). Second, the pK(a,i)(mix) values were recalculated to thermodynamic pK(a)s using the Debye-Huckel theory. Thermodynamic pK(a) of carboxyl groups was estimated to be in the range of 2.5-3.3 for C-terminal amino acids of the above peptides, and 5.2 for glutamic acid in the middle of peptide chain; pK(a) of imid

Název v anglickém jazyce

Determination of acidity constants and ionic mobilities of polyprotic peptide hormones by CZE

Popis výsledku anglicky

CZE has been applied to determination of thermodynamic acidity constants (pK(a)) of ionogenic groups and actual ionic mobilities of polyprotic peptides-synthetic human and salmon gonadotropin-releasing hormones and their derivatives and fragments. First,the mixed acidity constants, pK(a,i)(mix), of ionogenic groups, and actual ionic mobilities, m(i), of gonadotropin-releasing hormone peptides were determined by nonlinear regression analysis of pH dependence of their effective electrophoretic mobilities. The effective mobilities were measured by CZE in a series of BGEs within a broad pH range (1.80-12.10), at constant ionic strength (25 mM) and reference temperature (25 degrees C). Second, the pK(a,i)(mix) values were recalculated to thermodynamic pK(a)s using the Debye-Huckel theory. Thermodynamic pK(a) of carboxyl groups was estimated to be in the range of 2.5-3.3 for C-terminal amino acids of the above peptides, and 5.2 for glutamic acid in the middle of peptide chain; pK(a) of imid

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

34

Číslo periodika v rámci svazku

18

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

2655-2665

Kód UT WoS článku

000327590900008

EID výsledku v databázi Scopus

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