Estimation of acidity constants, ionic mobilities and charges of antimicrobial peptides by capillary electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00470552" target="_blank" >RIV/61388963:_____/16:00470552 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1002/elps.201600342" target="_blank" >http://dx.doi.org/10.1002/elps.201600342</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201600342" target="_blank" >10.1002/elps.201600342</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Estimation of acidity constants, ionic mobilities and charges of antimicrobial peptides by capillary electrophoresis

Popis výsledku v původním jazyce

Capillary electrophoresis (CE) was employed for the determination of thermodynamic acidity constants (pK(a)) and actual ionic mobilities of polycationic antimicrobial peptides (AMPs). The effective electrophoretic mobilities of AMPs were measured by CE in a series of the background electrolytes within a wide pH range (2.00-12.25), at constant ionic strength (25mM) and ambient temperature, using polybrene coated fused silica capillaries to suppress sorption of cationic AMPs to the capillary wall. Eventually, Haarhoff-Van der Linde peak fitting function was used for the determination of correct migration times of some AMPs peaks that were distorted by electromigration dispersion. The measured effective mobilities were corrected to 25 degrees C. Mixed acidity constants, pK(a,i)(mix), and actual ionic mobilities, m(i), of AMPs were determined by the nonlinear regression analysis of pH dependence of their effective mobilities. The pK(a,i)(mix) values were recalculated to thermodynamic pK(a)s using the Debye-Huckel theory. Thermodynamic pK(a) of imidazolium group of histidine residues was found to be in the range 3.72-4.98, pK(a) of alpha-NH3+ group was in the range 6.14-6.93, and pK(a) of epsilon-NH3+ group of lysine spanned the interval 7.26-9.84, depending on the particular amino acid sequence of the AMPs. Actual ionic mobilities of AMPs with positive charges from one to six elementary units achieved values (9.8-36.5) x 10(-9) m(2)V(-1)s(-1).

Název v anglickém jazyce

Estimation of acidity constants, ionic mobilities and charges of antimicrobial peptides by capillary electrophoresis

Popis výsledku anglicky

Capillary electrophoresis (CE) was employed for the determination of thermodynamic acidity constants (pK(a)) and actual ionic mobilities of polycationic antimicrobial peptides (AMPs). The effective electrophoretic mobilities of AMPs were measured by CE in a series of the background electrolytes within a wide pH range (2.00-12.25), at constant ionic strength (25mM) and ambient temperature, using polybrene coated fused silica capillaries to suppress sorption of cationic AMPs to the capillary wall. Eventually, Haarhoff-Van der Linde peak fitting function was used for the determination of correct migration times of some AMPs peaks that were distorted by electromigration dispersion. The measured effective mobilities were corrected to 25 degrees C. Mixed acidity constants, pK(a,i)(mix), and actual ionic mobilities, m(i), of AMPs were determined by the nonlinear regression analysis of pH dependence of their effective mobilities. The pK(a,i)(mix) values were recalculated to thermodynamic pK(a)s using the Debye-Huckel theory. Thermodynamic pK(a) of imidazolium group of histidine residues was found to be in the range 3.72-4.98, pK(a) of alpha-NH3+ group was in the range 6.14-6.93, and pK(a) of epsilon-NH3+ group of lysine spanned the interval 7.26-9.84, depending on the particular amino acid sequence of the AMPs. Actual ionic mobilities of AMPs with positive charges from one to six elementary units achieved values (9.8-36.5) x 10(-9) m(2)V(-1)s(-1).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

<a href="/cs/project/GA15-01948S" target="_blank" >GA15-01948S: Kapilární elektromigrační techniky využívající afinitní selektory a smart-polymery pro analýzu a studium vlastností a interakcí biomolekul.</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

—

Svazek periodika

37

Číslo periodika v rámci svazku

23/24

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

10

Strana od-do

3186-3195

Kód UT WoS článku

000392421400019

EID výsledku v databázi Scopus

2-s2.0-84991671385