Study of deoxyribonucleic acid-ligand interactions by partial filling affinity capillary electrophoresis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F14%3A00430010" target="_blank" >RIV/61388963:_____/14:00430010 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/14:10323802

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2014.04.061" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2014.04.061</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2014.04.061" target="_blank" >10.1016/j.chroma.2014.04.061</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Study of deoxyribonucleic acid-ligand interactions by partial filling affinity capillary electrophoresis

Popis výsledku v původním jazyce

In this work, a new partial filling affinity capillary electrophoresis (PF-ACE) method has been developed and applied to investigation of non-covalent molecular interactions between double stranded DNA oligonucleotide (Dickerson dodecamer) and classicalDNA intercalator ligand-ethidiumbromide (EtBr) or oligophenylene derivatives-based potential new type of DNA ligands. Binding constants of DNA-ligand complexes were determined from the dependence of migration time changes of DNA oligomer (applied as analyte) on the length of ligand zones introduced beforehand as plugs of various lengths (0-75 mm with 12.5 mm step) in hydroxypropylcellulose coated fused silica capillary of 50/375 mu m I.D./O.D. and 400/300 mm total/effective length. PF-ACE experiments were performed in two background electrolytes, Tris-borate, pH 8.0, ionic strength 14.3 mM (BGE1), and sodium phosphate, pH 7.5, ionic strength 133 mM (BGE2). Binding constants of DNA-EtBr complex (ca 15300 L/mol in the BGE1 and 4200 L/mol

Název v anglickém jazyce

Study of deoxyribonucleic acid-ligand interactions by partial filling affinity capillary electrophoresis

Popis výsledku anglicky

In this work, a new partial filling affinity capillary electrophoresis (PF-ACE) method has been developed and applied to investigation of non-covalent molecular interactions between double stranded DNA oligonucleotide (Dickerson dodecamer) and classicalDNA intercalator ligand-ethidiumbromide (EtBr) or oligophenylene derivatives-based potential new type of DNA ligands. Binding constants of DNA-ligand complexes were determined from the dependence of migration time changes of DNA oligomer (applied as analyte) on the length of ligand zones introduced beforehand as plugs of various lengths (0-75 mm with 12.5 mm step) in hydroxypropylcellulose coated fused silica capillary of 50/375 mu m I.D./O.D. and 400/300 mm total/effective length. PF-ACE experiments were performed in two background electrolytes, Tris-borate, pH 8.0, ionic strength 14.3 mM (BGE1), and sodium phosphate, pH 7.5, ionic strength 133 mM (BGE2). Binding constants of DNA-EtBr complex (ca 15300 L/mol in the BGE1 and 4200 L/mol

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

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Svazek periodika

1349

Číslo periodika v rámci svazku

Jul

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

116-121

Kód UT WoS článku

000337851000014

EID výsledku v databázi Scopus

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