LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00507271" target="_blank" >RIV/61388963:_____/19:00507271 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/19:00522861 RIV/00216208:11110/19:10395894 RIV/60461373:22310/19:43919275

Výsledek na webu

<a href="https://www.nature.com/articles/s41598-019-45079-1" target="_blank" >https://www.nature.com/articles/s41598-019-45079-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s41598-019-45079-1" target="_blank" >10.1038/s41598-019-45079-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion

Popis výsledku v původním jazyce

The mechanism of action of various viruses has been the primary focus of many studies.Yet, the data on RNA modifications in any type of virus are scarce. Methods for the sensitive analysis of RNA modifications have been developed only recently and they have not been applied to viruses. In particular, the RNA composition of HIV-1 virions has never been determined with sufficiently exact methods. Here, we reveal that the RNA of HIV-1 virions contains surprisingly high amount of the 1-methyladenosine. We are the first to use a liquid chromatography-mass spectrometry analysis (LC/MS) of virion RNA, which we combined with nil-A profiling and deep sequencing. We found that m(1)A was present in the tRNA, but not in the genomic HIV-1 RNA and the abundant 7SL RNA. We were able to calculate that an HIV-1 virion contains per 2 copies of genomic RNA and 14 copies of 7SL RNA also 770 copies of tRNA, which is approximately 10 times more than thus far expected. These new insights into the composition of the HIV-1 virion can help in future studies to identify the role of nonprimer tRNAs in retroviruses. Moreover, we present a promising new tool for studying the compositions of virions.

Název v anglickém jazyce

LC/MS analysis and deep sequencing reveal the accurate RNA composition in the HIV-1 virion

Popis výsledku anglicky

The mechanism of action of various viruses has been the primary focus of many studies.Yet, the data on RNA modifications in any type of virus are scarce. Methods for the sensitive analysis of RNA modifications have been developed only recently and they have not been applied to viruses. In particular, the RNA composition of HIV-1 virions has never been determined with sufficiently exact methods. Here, we reveal that the RNA of HIV-1 virions contains surprisingly high amount of the 1-methyladenosine. We are the first to use a liquid chromatography-mass spectrometry analysis (LC/MS) of virion RNA, which we combined with nil-A profiling and deep sequencing. We found that m(1)A was present in the tRNA, but not in the genomic HIV-1 RNA and the abundant 7SL RNA. We were able to calculate that an HIV-1 virion contains per 2 copies of genomic RNA and 14 copies of 7SL RNA also 770 copies of tRNA, which is approximately 10 times more than thus far expected. These new insights into the composition of the HIV-1 virion can help in future studies to identify the role of nonprimer tRNAs in retroviruses. Moreover, we present a promising new tool for studying the compositions of virions.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Scientific Reports

ISSN

2045-2322

e-ISSN

—

Svazek periodika

9

Číslo periodika v rámci svazku

Jun 18

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

10

Strana od-do

8697

Kód UT WoS článku

000471868600012

EID výsledku v databázi Scopus

2-s2.0-85067545841