Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F22%3A00559536" target="_blank" >RIV/61388963:_____/22:00559536 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/22:00559536 RIV/60461373:22310/22:43925547 RIV/60076658:12310/22:43904907 RIV/00216208:11110/22:10445458 RIV/00216224:14740/22:00128475

Výsledek na webu

<a href="https://doi.org/10.1002/cbic.202200281" target="_blank" >https://doi.org/10.1002/cbic.202200281</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/cbic.202200281" target="_blank" >10.1002/cbic.202200281</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions

Popis výsledku v původním jazyce

The Picornavirales include viruses that infect vertebrates, insects, and plants. It was believed that they pack only their genomic mRNA in the particles, thus, we envisaged these viruses as excellent model systems for studies of mRNA modifications. We used LC–MS to analyze digested RNA isolated from particles of the sacbrood and deformed wing iflaviruses as well as of the echovirus 18 and rhinovirus 2 picornaviruses. Whereas in the picornavirus RNAs we detected only N6-methyladenosine and 2’-O-methylated nucleosides, the iflavirus RNAs contained a wide range of methylated nucleosides, such as 1-methyladenosine (m1A) and 5-methylcytidine (m5C). Mapping of m1A and m5C through RNA sequencing of the SBV and DWV RNAs revealed the presence of tRNA molecules. Both modifications were detected only in tRNA. Further analysis revealed that tRNAs are present in form of 3’ and 5’ fragments and they are packed selectively. Moreover, these tRNAs are typically packed by other viruses.

Název v anglickém jazyce

Honeybee Iflaviruses Pack Specific tRNA Fragments from Host Cells in Their Virions

Popis výsledku anglicky

The Picornavirales include viruses that infect vertebrates, insects, and plants. It was believed that they pack only their genomic mRNA in the particles, thus, we envisaged these viruses as excellent model systems for studies of mRNA modifications. We used LC–MS to analyze digested RNA isolated from particles of the sacbrood and deformed wing iflaviruses as well as of the echovirus 18 and rhinovirus 2 picornaviruses. Whereas in the picornavirus RNAs we detected only N6-methyladenosine and 2’-O-methylated nucleosides, the iflavirus RNAs contained a wide range of methylated nucleosides, such as 1-methyladenosine (m1A) and 5-methylcytidine (m5C). Mapping of m1A and m5C through RNA sequencing of the SBV and DWV RNAs revealed the presence of tRNA molecules. Both modifications were detected only in tRNA. Further analysis revealed that tRNAs are present in form of 3’ and 5’ fragments and they are packed selectively. Moreover, these tRNAs are typically packed by other viruses.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chembiochem

ISSN

1439-4227

e-ISSN

1439-7633

Svazek periodika

23

Číslo periodika v rámci svazku

17

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

e202200281

Kód UT WoS článku

000828265300001

EID výsledku v databázi Scopus

2-s2.0-85134532067