No magnesium is needed for binding of the stimulator of interferon genes to cyclic dinucleotides

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F19%3A00508878" target="_blank" >RIV/61388963:_____/19:00508878 - isvavai.cz</a>

Výsledek na webu

<a href="http://scripts.iucr.org/cgi-bin/paper?S2053230X19010999" target="_blank" >http://scripts.iucr.org/cgi-bin/paper?S2053230X19010999</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1107/S2053230X19010999" target="_blank" >10.1107/S2053230X19010999</a>

Jazyk výsledku

angličtina

Název v původním jazyce

No magnesium is needed for binding of the stimulator of interferon genes to cyclic dinucleotides

Popis výsledku v původním jazyce

Stimulator of interferon genes (STING) binds cyclic dinucleotides (CDNs), which induce a large conformational change of the protein. The structural basis of activation of STING by CDNs is rather well understood. Unliganded STING forms an open dimer that undergoes a large conformational change (similar to 10 angstrom) to a closed conformation upon the binding of a CDN molecule. This event activates downstream effectors of STING and subsequently leads to activation of the type 1 interferon response. However, a previously solved structure of STING with 3',3'-c-di-GMP shows Mg atoms mediating the interaction of STING with this CDN. Here, it is shown that no Mg atoms are needed for this interaction, in fact, magnesium can in some cases obstruct the binding of a CDN to STING.

Název v anglickém jazyce

No magnesium is needed for binding of the stimulator of interferon genes to cyclic dinucleotides

Popis výsledku anglicky

Stimulator of interferon genes (STING) binds cyclic dinucleotides (CDNs), which induce a large conformational change of the protein. The structural basis of activation of STING by CDNs is rather well understood. Unliganded STING forms an open dimer that undergoes a large conformational change (similar to 10 angstrom) to a closed conformation upon the binding of a CDN molecule. This event activates downstream effectors of STING and subsequently leads to activation of the type 1 interferon response. However, a previously solved structure of STING with 3',3'-c-di-GMP shows Mg atoms mediating the interaction of STING with this CDN. Here, it is shown that no Mg atoms are needed for this interaction, in fact, magnesium can in some cases obstruct the binding of a CDN to STING.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/EF16_019%2F0000729" target="_blank" >EF16_019/0000729: Chemická biologie pro vývoj nových terapií</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Acta Crystallographica Section F - Structural Biology Communications

ISSN

2053-230X

e-ISSN

—

Svazek periodika

75

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

593-598

Kód UT WoS článku

000484148400004

EID výsledku v databázi Scopus

2-s2.0-85071736712