Capillary electrophoretic profiling of in‐bone tryptic digests of proteins as a potential tool for the detection of inflammatory states in oral surgery

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00532117" target="_blank" >RIV/61388963:_____/20:00532117 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/20:10417285 RIV/60461373:22340/20:43921485

Výsledek na webu

<a href="https://doi.org/10.1002/jssc.202000718" target="_blank" >https://doi.org/10.1002/jssc.202000718</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.202000718" target="_blank" >10.1002/jssc.202000718</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Capillary electrophoretic profiling of in‐bone tryptic digests of proteins as a potential tool for the detection of inflammatory states in oral surgery

Popis výsledku v původním jazyce

The commonly used histological assessment of pathological states of alveolar bone tissues in oral surgery needs laborious and time‐consuming processing by an experienced histologist. Therefore, a simpler and faster methodology is required in this field. Following this demand, this paper reports a straightforward approach using the tryptic cleavage of proteins directly in bone without its demineralization, followed by the capillary electrophoresis‐ultraviolet detection profiling of the yielded protein digest. Cleavage‐derived peptides were separated by capillary electrophoresis in acidic background electrolytes, pH 2.01–2.54. The best resolution of peptide fragments with the highest peak capacity was achieved in the background electrolyte composed of 55 mM H3PO4, 14 mM tris(hydroxymethyl)aminomethan, pH 2.01. The differences in the obtained capillary electrophoresis‐ultraviolet detection profiles with characteristic patterns for particular bone samples were subsequently discriminated by linear discriminant analysis over principal components. This approach was first verified on porcine bone tissues as model samples, jawbone and calf bone tissues could be discriminated with an accuracy of 100%. Subsequently, the method was capable of differentiating unequivocally between human healthy and inflammatory alveolar bone tissues obtained from oral surgery. This procedure seems to be promising as complement or even an alternative to the traditional histological discrimination between healthy and inflammatory bone tissues in oral surgery.

Název v anglickém jazyce

Capillary electrophoretic profiling of in‐bone tryptic digests of proteins as a potential tool for the detection of inflammatory states in oral surgery

Popis výsledku anglicky

The commonly used histological assessment of pathological states of alveolar bone tissues in oral surgery needs laborious and time‐consuming processing by an experienced histologist. Therefore, a simpler and faster methodology is required in this field. Following this demand, this paper reports a straightforward approach using the tryptic cleavage of proteins directly in bone without its demineralization, followed by the capillary electrophoresis‐ultraviolet detection profiling of the yielded protein digest. Cleavage‐derived peptides were separated by capillary electrophoresis in acidic background electrolytes, pH 2.01–2.54. The best resolution of peptide fragments with the highest peak capacity was achieved in the background electrolyte composed of 55 mM H3PO4, 14 mM tris(hydroxymethyl)aminomethan, pH 2.01. The differences in the obtained capillary electrophoresis‐ultraviolet detection profiles with characteristic patterns for particular bone samples were subsequently discriminated by linear discriminant analysis over principal components. This approach was first verified on porcine bone tissues as model samples, jawbone and calf bone tissues could be discriminated with an accuracy of 100%. Subsequently, the method was capable of differentiating unequivocally between human healthy and inflammatory alveolar bone tissues obtained from oral surgery. This procedure seems to be promising as complement or even an alternative to the traditional histological discrimination between healthy and inflammatory bone tissues in oral surgery.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Svazek periodika

43

Číslo periodika v rámci svazku

20

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

11

Strana od-do

3949-3959

Kód UT WoS článku

000562972900001

EID výsledku v databázi Scopus

2-s2.0-85089782415