Directionality of light absorption and emission in representative fluorescent proteins

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00537175" target="_blank" >RIV/61388963:_____/20:00537175 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/20:00537547 RIV/68378050:_____/20:00537547

Výsledek na webu

<a href="https://doi.org/10.1073/pnas.2017379117" target="_blank" >https://doi.org/10.1073/pnas.2017379117</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1073/pnas.2017379117" target="_blank" >10.1073/pnas.2017379117</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Directionality of light absorption and emission in representative fluorescent proteins

Popis výsledku v původním jazyce

Fluorescent proteins have been used extensively in many areas of life sciences. Many of their applications rely on their various biophysical properties, such as excitation and emission wavelengths, excited state lifetimes, or sensitivity to the molecular environment. One aspect of fluorescent proteins that has remained largely neglected is the directionality of their optical properties. In the present work, we describe our experimental determination of the directionality of light absorption and emission in several commonly used fluorescent proteins. Our findings improve our understanding of fundamental properties of fluorescent proteins, and expand the possibilities of development and applications of genetically encoded fluorescent probes.

Název v anglickém jazyce

Directionality of light absorption and emission in representative fluorescent proteins

Popis výsledku anglicky

Fluorescent proteins have been used extensively in many areas of life sciences. Many of their applications rely on their various biophysical properties, such as excitation and emission wavelengths, excited state lifetimes, or sensitivity to the molecular environment. One aspect of fluorescent proteins that has remained largely neglected is the directionality of their optical properties. In the present work, we describe our experimental determination of the directionality of light absorption and emission in several commonly used fluorescent proteins. Our findings improve our understanding of fundamental properties of fluorescent proteins, and expand the possibilities of development and applications of genetically encoded fluorescent probes.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

<a href="/cs/project/EF16_019%2F0000729" target="_blank" >EF16_019/0000729: Chemická biologie pro vývoj nových terapií</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Proceedings of the National Academy of Sciences of the United States of America

ISSN

0027-8424

e-ISSN

—

Svazek periodika

117

Číslo periodika v rámci svazku

51

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

32395-32401

Kód UT WoS článku

000601315200031

EID výsledku v databázi Scopus

2-s2.0-85098213266