A ubiquitous disordered protein interaction module orchestrates transcription elongation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F21%3A00548921" target="_blank" >RIV/61388963:_____/21:00548921 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/21:00548921 RIV/00216208:11310/21:10439304

Výsledek na webu

<a href="https://doi.org/10.1126/science.abe2913" target="_blank" >https://doi.org/10.1126/science.abe2913</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1126/science.abe2913" target="_blank" >10.1126/science.abe2913</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A ubiquitous disordered protein interaction module orchestrates transcription elongation

Popis výsledku v původním jazyce

During eukaryotic transcription elongation, RNA polymerase II (RNAP2) is regulated by a chorus of factors. Here, we identified a common binary interaction module consisting of TFIIS N-terminal domains (TNDs) and natively unstructured TND-interacting motifs (TIMs). This module was conserved among the elongation machinery and linked complexes including transcription factor TFIIS, Mediator, super elongation complex, elongin, IWS1, SPT6, PP1-PNUTS phosphatase, H3K36me3 readers, and other factors. Using nuclear magnetic resonance, live-cell microscopy, and mass spectrometry, we revealed the structural basis for these interactions and found that TND-TIM sequences were necessary and sufficient to induce strong and specific colocalization in the crowded nuclear environment. Disruption of a single TIM in IWS1 induced robust changes in gene expression and RNAP2 elongation dynamics, which underscores the functional importance of TND-TIM surfaces for transcription elongation.

Název v anglickém jazyce

A ubiquitous disordered protein interaction module orchestrates transcription elongation

Popis výsledku anglicky

During eukaryotic transcription elongation, RNA polymerase II (RNAP2) is regulated by a chorus of factors. Here, we identified a common binary interaction module consisting of TFIIS N-terminal domains (TNDs) and natively unstructured TND-interacting motifs (TIMs). This module was conserved among the elongation machinery and linked complexes including transcription factor TFIIS, Mediator, super elongation complex, elongin, IWS1, SPT6, PP1-PNUTS phosphatase, H3K36me3 readers, and other factors. Using nuclear magnetic resonance, live-cell microscopy, and mass spectrometry, we revealed the structural basis for these interactions and found that TND-TIM sequences were necessary and sufficient to induce strong and specific colocalization in the crowded nuclear environment. Disruption of a single TIM in IWS1 induced robust changes in gene expression and RNAP2 elongation dynamics, which underscores the functional importance of TND-TIM surfaces for transcription elongation.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Science

ISSN

0036-8075

e-ISSN

1095-9203

Svazek periodika

374

Číslo periodika v rámci svazku

6571

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

1113-1121

Kód UT WoS článku

000725668600044

EID výsledku v databázi Scopus

2-s2.0-85120034108