Loss of UCP1 function augments recruitment of futile lipid cycling for thermogenesis in murine brown fat

Kód výsledku v IS VaVaI

RIV/61388963:_____/22:00558033 - isvavai.cz

Nalezeny alternativní kódy

RIV/67985823:_____/22:00558033

Výsledek na webu

https://doi.org/10.1016/j.molmet.2022.101499

DOI - Digital Object Identifier

10.1016/j.molmet.2022.101499

Jazyk výsledku

angličtina

Název v původním jazyce

Loss of UCP1 function augments recruitment of futile lipid cycling for thermogenesis in murine brown fat

Popis výsledku v původním jazyce

Objective:Classical ATP-independent non-shivering thermogenesis enabled by uncoupling protein 1 (UCP1) in brown adipose tissue (BAT) is activated, but not essential for survival, in the cold. It has long been suspected that futile ATP-consuming substrate cycles also contribute to thermogenesis and can partially compensate for the genetic ablation of UCP1 in mouse models. Futile ATP-dependent thermogenesis could thereby enable survival in the cold even when brown fat is less abundant or missing.Methods:In this study, we explore different potential sources of UCP1-independent thermogenesis and identify a futile ATP-consuming triglyceride/fatty acid cycle as the main contributor to cellular heat production in brown adipocytes lacking UCP1. We uncover the mechanism on a molecular level and pinpoint the key enzymes involved using pharmacological and genetic interference.Results:ATGL is the most important lipase in terms of releasing fatty acids from lipid droplets, while DGAT1 accounts for the majority of fatty acid re-esterification in UCP1-ablated brown adipocytes. Furthermore, we demonstrate that chronic cold exposure causes a pronounced remodeling of adipose tissues and leads to the recruitment of lipid cycling capacity specifically in BAT of UCP1-knockout mice, possibly fueled by fatty acids from white fat. Quantification of triglyceride/fatty acid cycling clearly shows that UCP1-ablated animals significantly increase turnover rates at room temperature and below.Conclusion:Our results suggest an important role for futile lipid cycling in adaptive thermogenesis and total energy expenditure.

Název v anglickém jazyce

Loss of UCP1 function augments recruitment of futile lipid cycling for thermogenesis in murine brown fat

Popis výsledku anglicky

Objective:Classical ATP-independent non-shivering thermogenesis enabled by uncoupling protein 1 (UCP1) in brown adipose tissue (BAT) is activated, but not essential for survival, in the cold. It has long been suspected that futile ATP-consuming substrate cycles also contribute to thermogenesis and can partially compensate for the genetic ablation of UCP1 in mouse models. Futile ATP-dependent thermogenesis could thereby enable survival in the cold even when brown fat is less abundant or missing.Methods:In this study, we explore different potential sources of UCP1-independent thermogenesis and identify a futile ATP-consuming triglyceride/fatty acid cycle as the main contributor to cellular heat production in brown adipocytes lacking UCP1. We uncover the mechanism on a molecular level and pinpoint the key enzymes involved using pharmacological and genetic interference.Results:ATGL is the most important lipase in terms of releasing fatty acids from lipid droplets, while DGAT1 accounts for the majority of fatty acid re-esterification in UCP1-ablated brown adipocytes. Furthermore, we demonstrate that chronic cold exposure causes a pronounced remodeling of adipose tissues and leads to the recruitment of lipid cycling capacity specifically in BAT of UCP1-knockout mice, possibly fueled by fatty acids from white fat. Quantification of triglyceride/fatty acid cycling clearly shows that UCP1-ablated animals significantly increase turnover rates at room temperature and below.Conclusion:Our results suggest an important role for futile lipid cycling in adaptive thermogenesis and total energy expenditure.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

GA18-04483S: Úloha bílé tukové tkáně v termogenní odpovědi

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Metabolism

ISSN

2212-8778

e-ISSN

2212-8778

Svazek periodika

61

Číslo periodika v rámci svazku

July

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

21

Strana od-do

101499

Kód UT WoS článku

000804540600004

EID výsledku v databázi Scopus

2-s2.0-85129587717