Adipose tissue-specific ablation of PGC-1 beta impairs thermogenesis in brown fat

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985823%3A_____%2F22%3A00557788" target="_blank" >RIV/67985823:_____/22:00557788 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1242/dmm.049223" target="_blank" >https://doi.org/10.1242/dmm.049223</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1242/dmm.049223" target="_blank" >10.1242/dmm.049223</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Adipose tissue-specific ablation of PGC-1 beta impairs thermogenesis in brown fat

Popis výsledku v původním jazyce

Impaired thermogenesis observed in mice with whole-body ablation of peroxisome proliferator-activated receptor-gamma coactivator-1 beta (PGC-1 beta, officially known as PPARGC1B) may result from impaired brown fat (brown adipose tissue, BAT) function, but other mechanism(s) could be involved. Here, using adipose-specific PGC-1 beta knockout mice (PGC-1 beta-AT-KO mice) we aimed to learn whether specific PGC-1 beta ablation in adipocytes is sufficient to drive cold sensitivity. Indeed, we found that warm-adapted (30 degrees C) mutant mice were relatively sensitive to acute cold exposure (6 degrees C). When these mice were subjected to cold exposure for 7 days (7-day-CE), adrenergic stimulation of their metabolism was impaired, despite similar levels of thermogenic uncoupling protein 1 in BAT in PGC-1 beta-AT-KO and wild-type mice. Gene expression in BAT of mutant mice suggested a compensatory increase in lipid metabolism to counteract the thermogenic defect. Interestingly, a reduced number of contacts between mitochondria and lipid droplets associated with low levels of L-form of optic atrophy 1 was found in BAT of PGC-1 beta-AT-KO mice. These genotypic differences were observed in warm-adapted mutant mice, but they were partially masked by 7-day-CE. Collectively, our results suggest a role for PGC-1 beta in controlling BAT lipid metabolism and thermogenesis.

Název v anglickém jazyce

Adipose tissue-specific ablation of PGC-1 beta impairs thermogenesis in brown fat

Popis výsledku anglicky

Impaired thermogenesis observed in mice with whole-body ablation of peroxisome proliferator-activated receptor-gamma coactivator-1 beta (PGC-1 beta, officially known as PPARGC1B) may result from impaired brown fat (brown adipose tissue, BAT) function, but other mechanism(s) could be involved. Here, using adipose-specific PGC-1 beta knockout mice (PGC-1 beta-AT-KO mice) we aimed to learn whether specific PGC-1 beta ablation in adipocytes is sufficient to drive cold sensitivity. Indeed, we found that warm-adapted (30 degrees C) mutant mice were relatively sensitive to acute cold exposure (6 degrees C). When these mice were subjected to cold exposure for 7 days (7-day-CE), adrenergic stimulation of their metabolism was impaired, despite similar levels of thermogenic uncoupling protein 1 in BAT in PGC-1 beta-AT-KO and wild-type mice. Gene expression in BAT of mutant mice suggested a compensatory increase in lipid metabolism to counteract the thermogenic defect. Interestingly, a reduced number of contacts between mitochondria and lipid droplets associated with low levels of L-form of optic atrophy 1 was found in BAT of PGC-1 beta-AT-KO mice. These genotypic differences were observed in warm-adapted mutant mice, but they were partially masked by 7-day-CE. Collectively, our results suggest a role for PGC-1 beta in controlling BAT lipid metabolism and thermogenesis.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30202 - Endocrinology and metabolism (including diabetes, hormones)

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Disease Models & Mechanisms

ISSN

1754-8403

e-ISSN

1754-8411

Svazek periodika

15

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

11

Strana od-do

dmm049223

Kód UT WoS článku

000796546700007

EID výsledku v databázi Scopus

2-s2.0-85128801831