Metabolomic Study of Aging in fa/fa Rats: Multiplatform Urine and Serum Analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F23%3A00575321" target="_blank" >RIV/61388963:_____/23:00575321 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/23:00572141 RIV/61388971:_____/23:00575321 RIV/00216208:11110/23:10465309 RIV/49777513:23520/23:43971197

Výsledek na webu

<a href="https://www.mdpi.com/2218-1989/13/4/552" target="_blank" >https://www.mdpi.com/2218-1989/13/4/552</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/metabo13040552" target="_blank" >10.3390/metabo13040552</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Metabolomic Study of Aging in fa/fa Rats: Multiplatform Urine and Serum Analysis

Popis výsledku v původním jazyce

Zucker fatty (fa/fa) rats represent a well-established and widely used model of genetic obesity. Because previous metabolomic studies have only been published for young fa/fa rats up to 20 weeks of age, which can be considered early maturity in male fa/fa rats, the aim of our work was to extend the metabolomic characterization to significantly older animals. Therefore, the urinary profiles of obese fa/fa rats and their lean controls were monitored using untargeted NMR metabolomics between 12 and 40 weeks of age. At the end of the experiment, the rats were also characterized by NMR and LC-MS serum analysis, which was supplemented by a targeted LC-MS analysis of serum bile acids and neurotransmitters. The urine analysis showed that most of the characteristic differences detected in young obese fa/fa rats persisted throughout the experiment, primarily through a decrease in microbial co-metabolite levels, the upregulation of the citrate cycle, and changes in nicotinamide metabolism compared with the age-related controls. The serum of 40-week-old obese rats showed a reduction in several bile acid conjugates and an increase in serotonin. Our study demonstrated that the fa/fa model of genetic obesity is stable up to 40 weeks of age and is therefore suitable for long-term experiments.

Název v anglickém jazyce

Metabolomic Study of Aging in fa/fa Rats: Multiplatform Urine and Serum Analysis

Popis výsledku anglicky

Zucker fatty (fa/fa) rats represent a well-established and widely used model of genetic obesity. Because previous metabolomic studies have only been published for young fa/fa rats up to 20 weeks of age, which can be considered early maturity in male fa/fa rats, the aim of our work was to extend the metabolomic characterization to significantly older animals. Therefore, the urinary profiles of obese fa/fa rats and their lean controls were monitored using untargeted NMR metabolomics between 12 and 40 weeks of age. At the end of the experiment, the rats were also characterized by NMR and LC-MS serum analysis, which was supplemented by a targeted LC-MS analysis of serum bile acids and neurotransmitters. The urine analysis showed that most of the characteristic differences detected in young obese fa/fa rats persisted throughout the experiment, primarily through a decrease in microbial co-metabolite levels, the upregulation of the citrate cycle, and changes in nicotinamide metabolism compared with the age-related controls. The serum of 40-week-old obese rats showed a reduction in several bile acid conjugates and an increase in serotonin. Our study demonstrated that the fa/fa model of genetic obesity is stable up to 40 weeks of age and is therefore suitable for long-term experiments.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30105 - Physiology (including cytology)

Projekt

<a href="/cs/project/GA20-00546S" target="_blank" >GA20-00546S: Souvislost obezity, diabetu a neurodegenerace: nový terapeutický potenciál analogů peptidu uvolňujícího prolaktin</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Metabolites

ISSN

2218-1989

e-ISSN

2218-1989

Svazek periodika

13

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

20

Strana od-do

552

Kód UT WoS článku

000977112100001

EID výsledku v databázi Scopus

2-s2.0-85153742885