19F MRI In Vivo Monitoring of Gelatin-Based Hydrogels: 3D Scaffolds with Tunable Biodegradation toward Regenerative Medicine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00585751" target="_blank" >RIV/61388963:_____/24:00585751 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/24:00585751 RIV/00216208:11110/24:10482618 RIV/00064165:_____/24:10482618

Výsledek na webu

<a href="https://doi.org/10.1021/acs.chemmater.3c03321" target="_blank" >https://doi.org/10.1021/acs.chemmater.3c03321</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/acs.chemmater.3c03321" target="_blank" >10.1021/acs.chemmater.3c03321</a>

Jazyk výsledku

angličtina

Název v původním jazyce

19F MRI In Vivo Monitoring of Gelatin-Based Hydrogels: 3D Scaffolds with Tunable Biodegradation toward Regenerative Medicine

Popis výsledku v původním jazyce

Gelatin-based hydrogels emerged as promising biodegradable cell-compatible 3D-printable materials with tunable mechanical properties that serve tissue engineering and applications in regenerative medicine. Nevertheless, these materials are very challenging to monitor in vivo, which has hampered the further development of these materials and their translation into clinical practice. To overcome this limitation, we designed a cross-linked 3D-printable gelatin-based hydrogel endowed with poly[N-(2,2-difluoroethyl)acrylamide] (PDFEA). Such PDFEA-containing hydrogels can be monitored in vivo through fluorine-19 magnetic resonance imaging (19F MRI), which enables to monitor such implants in vivo and to assess their in vivo biodegradation kinetics. Herein, we prepared three different PDFEA-containing hydrogels with varying cross-linking degrees and studied their physicochemical properties (storage modulus, Young’s modulus, swelling ratio, in vitro degradation rate). Next, we administered these samples subcutaneously into mice and exploited 19F MRI to detect the biodegradation kinetics over 370 days. Hydrogels with a high cross-linking degree did not extensively degrade in vitro nor in vivo within the evaluated time frame. In contrast, hydrogels characterized by a low degree of cross-linking extensively degraded in vitro as well as in vivo (half-life of 228 ± 21 days). We demonstrated that endowing hydrogels with PDFEA enables monitoring of these hydrogels in vivo. Our results may become a benchmark in forthcoming studies of biodegradable hydrogels and the development of 19F MRI detectable gelatin-based hydrogels, paving the way toward their entry in clinical practice.

Název v anglickém jazyce

19F MRI In Vivo Monitoring of Gelatin-Based Hydrogels: 3D Scaffolds with Tunable Biodegradation toward Regenerative Medicine

Popis výsledku anglicky

Gelatin-based hydrogels emerged as promising biodegradable cell-compatible 3D-printable materials with tunable mechanical properties that serve tissue engineering and applications in regenerative medicine. Nevertheless, these materials are very challenging to monitor in vivo, which has hampered the further development of these materials and their translation into clinical practice. To overcome this limitation, we designed a cross-linked 3D-printable gelatin-based hydrogel endowed with poly[N-(2,2-difluoroethyl)acrylamide] (PDFEA). Such PDFEA-containing hydrogels can be monitored in vivo through fluorine-19 magnetic resonance imaging (19F MRI), which enables to monitor such implants in vivo and to assess their in vivo biodegradation kinetics. Herein, we prepared three different PDFEA-containing hydrogels with varying cross-linking degrees and studied their physicochemical properties (storage modulus, Young’s modulus, swelling ratio, in vitro degradation rate). Next, we administered these samples subcutaneously into mice and exploited 19F MRI to detect the biodegradation kinetics over 370 days. Hydrogels with a high cross-linking degree did not extensively degrade in vitro nor in vivo within the evaluated time frame. In contrast, hydrogels characterized by a low degree of cross-linking extensively degraded in vitro as well as in vivo (half-life of 228 ± 21 days). We demonstrated that endowing hydrogels with PDFEA enables monitoring of these hydrogels in vivo. Our results may become a benchmark in forthcoming studies of biodegradable hydrogels and the development of 19F MRI detectable gelatin-based hydrogels, paving the way toward their entry in clinical practice.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10404 - Polymer science

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Chemistry of Materials

ISSN

0897-4756

e-ISSN

1520-5002

Svazek periodika

36

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

4417-4425

Kód UT WoS článku

001226362400001

EID výsledku v databázi Scopus

2-s2.0-85191156450