Viral RNA-methyltransferases: function, structure and inhibition

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F24%3A00604159" target="_blank" >RIV/61388963:_____/24:00604159 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.xray.cz/setkani/abst2024/784.htm" target="_blank" >https://www.xray.cz/setkani/abst2024/784.htm</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Viral RNA-methyltransferases: function, structure and inhibition

Popis výsledku v původním jazyce

Methyltransferases (MTases) associated with coronaviruses, such as nsp10/16 and nsp14, are responsible for the final stages of RNA-cap formation within the cytoplasm. This cap is crucial for maintaining the stability of viral RNA and plays a pivotal role in evading the innate immune response. Uncapped RNA is swiftly detected by the innate immune system, triggering degradation and activating antiviral defenses. Consequently, the coronaviral MTases have become focal points of extensive scientific investigation. Recently, through the use of X-ray and cryo-electron microscopy techniques, the structures of both enzymes have been elucidated, even in complex with other components of the viral replication machinery. The application of high-throughput screening methods and the design of inhibitors guided by structural insights have resulted in the identification of potent inhibitors targeting these MTases. This talk critically examines the remarkable progress made in the field of coronaviral MTases since the onset of the COVID-19 pandemic. Additionally, it is worth noting that similar investigations into poxviruses' MTases have also contributed significantly to our understanding of viral replication and immune evasion strategies.

Název v anglickém jazyce

Viral RNA-methyltransferases: function, structure and inhibition

Popis výsledku anglicky

Methyltransferases (MTases) associated with coronaviruses, such as nsp10/16 and nsp14, are responsible for the final stages of RNA-cap formation within the cytoplasm. This cap is crucial for maintaining the stability of viral RNA and plays a pivotal role in evading the innate immune response. Uncapped RNA is swiftly detected by the innate immune system, triggering degradation and activating antiviral defenses. Consequently, the coronaviral MTases have become focal points of extensive scientific investigation. Recently, through the use of X-ray and cryo-electron microscopy techniques, the structures of both enzymes have been elucidated, even in complex with other components of the viral replication machinery. The application of high-throughput screening methods and the design of inhibitors guided by structural insights have resulted in the identification of potent inhibitors targeting these MTases. This talk critically examines the remarkable progress made in the field of coronaviral MTases since the onset of the COVID-19 pandemic. Additionally, it is worth noting that similar investigations into poxviruses' MTases have also contributed significantly to our understanding of viral replication and immune evasion strategies.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/LX22NPO5103" target="_blank" >LX22NPO5103: Národní institut virologie a bakteriologie</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů