Separation and analysis of proteins by capillary electromigration methods in the period mid-2021–2024

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F25%3A00638117" target="_blank" >RIV/61388963:_____/25:00638117 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1016/j.aca.2025.344323" target="_blank" >https://doi.org/10.1016/j.aca.2025.344323</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.aca.2025.344323" target="_blank" >10.1016/j.aca.2025.344323</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Separation and analysis of proteins by capillary electromigration methods in the period mid-2021–2024

Popis výsledku v původním jazyce

This review article presents an overview of the recent significant developments in the separation and analysis of intact proteins by capillary electromigration methods published in the period mid-2021–end-2024. The topics reported include sample preparation, prevention of protein adsorption on the inner fused silica capillary wall and control of electroosmotic flow, separation and analysis of proteins by distinct capillary electromigration methods, and various detection techniques online and offline coupled with these methods. Moreover, the most significant applications of CE methods in the areas of quality control of protein pharmaceuticals, determination of proteins in complex matrices, study of protein post-translational modifications and peptide mapping, investigation of protein interactions with other biomolecules, and determination of physicochemical characteristics of proteins are presented. Advantages and issues of CE methods and future trends of their developments are discussed.

Název v anglickém jazyce

Separation and analysis of proteins by capillary electromigration methods in the period mid-2021–2024

Popis výsledku anglicky

This review article presents an overview of the recent significant developments in the separation and analysis of intact proteins by capillary electromigration methods published in the period mid-2021–end-2024. The topics reported include sample preparation, prevention of protein adsorption on the inner fused silica capillary wall and control of electroosmotic flow, separation and analysis of proteins by distinct capillary electromigration methods, and various detection techniques online and offline coupled with these methods. Moreover, the most significant applications of CE methods in the areas of quality control of protein pharmaceuticals, determination of proteins in complex matrices, study of protein post-translational modifications and peptide mapping, investigation of protein interactions with other biomolecules, and determination of physicochemical characteristics of proteins are presented. Advantages and issues of CE methods and future trends of their developments are discussed.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytica Chimica Acta

ISSN

0003-2670

e-ISSN

1873-4324

Svazek periodika

1368

Číslo periodika v rámci svazku

September

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

29

Strana od-do

344323

Kód UT WoS článku

001540646600001

EID výsledku v databázi Scopus

2-s2.0-105008353136