Identifikace redukčních produktů bilirubinu kmene Clostridium perfringens izolovaného z novorozenecké fekální flóry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F06%3A00050802" target="_blank" >RIV/61388971:_____/06:00050802 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/06:00005072

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora

Popis výsledku v původním jazyce

The strain of Clostridium perfringens isolated from neonatal stools, capable of reducing bilirubin, was used in the study. Bacteria were incubated under anaerobic conditions with various native as well as synthetic bile pigments and their reduction products were analyzed by spectrophotometry, spectrofluorometry and mass spectrometry. A broad substrate activity was detected in the investigated strain of C. perfringens and a series of bilirubin reduction products was identified. Based on their physical?chemical properties, as well as mass spectra, end-catabolic bilirubin products were identified to belong to urobilinogen species. The reduction process, catalyzed enzymatically by the studied bacterial strain, does not proceed to stercobilinogen

Název v anglickém jazyce

Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora

Popis výsledku anglicky

The strain of Clostridium perfringens isolated from neonatal stools, capable of reducing bilirubin, was used in the study. Bacteria were incubated under anaerobic conditions with various native as well as synthetic bile pigments and their reduction products were analyzed by spectrophotometry, spectrofluorometry and mass spectrometry. A broad substrate activity was detected in the investigated strain of C. perfringens and a series of bilirubin reduction products was identified. Based on their physical?chemical properties, as well as mass spectra, end-catabolic bilirubin products were identified to belong to urobilinogen species. The reduction process, catalyzed enzymatically by the studied bacterial strain, does not proceed to stercobilinogen

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA310%2F02%2F1436" target="_blank" >GA310/02/1436: Izolace a charakterizace genu kodujícího bilirubinreduktázu z bakterie rodu Clostridium a její potenciální terapeutické využití</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences

ISSN

1570-0232

e-ISSN

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Svazek periodika

833

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

149-157

Kód UT WoS článku

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EID výsledku v databázi Scopus

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