Fosforylace podjednotky HsdR restrikčně-modifikačního enzymu Typu IA EcoKI

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Phosphorylation of Type IA restriction-modification complex enzyme EcoKI on the HsdR subunit

Popis výsledku v původním jazyce

Phosphorylation of Type I restriction-modification enzymes EcoKI, EcoAI, and EcoR124I ? representatives of IA, IB, and IC families, respectively ? was analyzed in vivo by immunoblotting of endogenous phosphoproteins isolated from Escherichia coli strainsharbouring the corresponding hsd genes, and in vitro by phosphorylation assay using protein kinase present in subcellular fractions of E. coli. From all three restriction-modification enzymes, the HsdR subunit of EcoKI system was the only subunit, whichwas phosphorylated. Further, we present evidence that HsdR is phosphorylated in vivo only when co-produced with HsdM and HsdS subunits as part of assembled EcoKI restriction endonuclease, while the individually produced HsdR subunit is not phosphorylated. In vitro phosphorylation of the HsdR subunit of purified EcoKI endonuclease occurs on Thr, and is strictly dependent on the addition of a catalytic amount of cytoplasmic fraction isolated from E. coli

Název v anglickém jazyce

Phosphorylation of Type IA restriction-modification complex enzyme EcoKI on the HsdR subunit

Popis výsledku anglicky

Phosphorylation of Type I restriction-modification enzymes EcoKI, EcoAI, and EcoR124I ? representatives of IA, IB, and IC families, respectively ? was analyzed in vivo by immunoblotting of endogenous phosphoproteins isolated from Escherichia coli strainsharbouring the corresponding hsd genes, and in vitro by phosphorylation assay using protein kinase present in subcellular fractions of E. coli. From all three restriction-modification enzymes, the HsdR subunit of EcoKI system was the only subunit, whichwas phosphorylated. Further, we present evidence that HsdR is phosphorylated in vivo only when co-produced with HsdM and HsdS subunits as part of assembled EcoKI restriction endonuclease, while the individually produced HsdR subunit is not phosphorylated. In vitro phosphorylation of the HsdR subunit of purified EcoKI endonuclease occurs on Thr, and is strictly dependent on the addition of a catalytic amount of cytoplasmic fraction isolated from E. coli

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FEMS Microbiology Letters

ISSN

0378-1097

e-ISSN

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Svazek periodika

270

Číslo periodika v rámci svazku

-

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

171-177

Kód UT WoS článku

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EID výsledku v databázi Scopus

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