The effect of the mutations in the HsdS polypeptide on function of the Type I restriction-modification system EcoR124I

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F09%3A00340965" target="_blank" >RIV/61388971:_____/09:00340965 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of the mutations in the HsdS polypeptide on function of the Type I restriction-modification system EcoR124I

Popis výsledku v původním jazyce

In the most complex Type I RM systems, restriction and modification activities are catalysed by one enzyme composed of three different subunits, which are encoded by the hsdR, hsdM and hsdS genes.The HsdS subunit plays a key role in the function of TypeI R-M enzymes being responsible for both the specific recognition of the target DNA and interaction with HsdM and HsdR subunits, while the HsdR subunit is representing the motor component of this enzyme being responsible for ATP-binding, ATP- hydrolysisand subsequent DNA translocation. We focused on identification of amino acids residues involved in subunit assembly of the Type I R-M enzyme EcoR124I

Název v anglickém jazyce

The effect of the mutations in the HsdS polypeptide on function of the Type I restriction-modification system EcoR124I

Popis výsledku anglicky

In the most complex Type I RM systems, restriction and modification activities are catalysed by one enzyme composed of three different subunits, which are encoded by the hsdR, hsdM and hsdS genes.The HsdS subunit plays a key role in the function of TypeI R-M enzymes being responsible for both the specific recognition of the target DNA and interaction with HsdM and HsdR subunits, while the HsdR subunit is representing the motor component of this enzyme being responsible for ATP-binding, ATP- hydrolysisand subsequent DNA translocation. We focused on identification of amino acids residues involved in subunit assembly of the Type I R-M enzyme EcoR124I

Druh

O - Ostatní výsledky

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA204%2F07%2F0325" target="_blank" >GA204/07/0325: Identifikace aminokyselinových zbytků podjednotek HsdS a HsdR nutných pro správné sestavení restrikčně-modifikačního enzymu Typu I EcoR124I</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů