Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F10%3A00346619" target="_blank" >RIV/61388971:_____/10:00346619 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/86652036:_____/10:00346619 RIV/60076658:12520/10:00011410

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L

Popis výsledku v původním jazyce

Damage to spermatozoa during cryopreservation is regarded as a major obstacle to the expansion of sperm storage technology. The authors used 2D PAA gel electrophoresis and MALDI-TOF to explore whether the protein profile of common carp (Cyprinus carpio)spermatozoa is affected by cryopreservation. 14 protein spots were significantly altered following cryopreservation. Eleven of these were identified: three as specific membrane proteins involved in membrane trafficking, organization, and cell movement; six as cytoplasmic enzymes involved in cell metabolism, oxidoreductase activity, and signal transduction; and transferrin. Based on these findings, the authors hypothesize that transferrin in cryopreserved sperm may protect spermatozoa against oxidative damage during the freeze-thaw process. Cryopreservation caused changes in spermatozoa protein profiles that may lead to decreased spermatozoa velocity, motility, fertilization success and to subsequent ova hatching rate.

Název v anglickém jazyce

Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L

Popis výsledku anglicky

Damage to spermatozoa during cryopreservation is regarded as a major obstacle to the expansion of sperm storage technology. The authors used 2D PAA gel electrophoresis and MALDI-TOF to explore whether the protein profile of common carp (Cyprinus carpio)spermatozoa is affected by cryopreservation. 14 protein spots were significantly altered following cryopreservation. Eleven of these were identified: three as specific membrane proteins involved in membrane trafficking, organization, and cell movement; six as cytoplasmic enzymes involved in cell metabolism, oxidoreductase activity, and signal transduction; and transferrin. Based on these findings, the authors hypothesize that transferrin in cryopreserved sperm may protect spermatozoa against oxidative damage during the freeze-thaw process. Cryopreservation caused changes in spermatozoa protein profiles that may lead to decreased spermatozoa velocity, motility, fertilization success and to subsequent ova hatching rate.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EI - Biotechnologie a bionika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Theriogenelogy

ISSN

0093-691X

e-ISSN

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Svazek periodika

74

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

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Kód UT WoS článku

000280341300009

EID výsledku v databázi Scopus

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