Detection of photosynthetic herbicides: Algal growth inhibition test vs. electrochemical photosystem II biosensor

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F11%3A00356190" target="_blank" >RIV/61388971:_____/11:00356190 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67179843:_____/11:00356190 RIV/67179843:_____/11:00371646 RIV/61388971:_____/11:00371646 RIV/44555601:13440/11:43881932 RIV/60076658:12520/11:43881312

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Detection of photosynthetic herbicides: Algal growth inhibition test vs. electrochemical photosystem II biosensor

Popis výsledku v původním jazyce

We compared a novel PSII-biosensor assay with a standard algal growth inhibition test for detection of photosynthetic herbicides?diuron, atrazine and isoproturon in liquid samples. To evaluate the convenience and sensitivity, values of the parameters EC50 and LOD and the duration of assays were compared. The biosensor assay was made with an electrochemical biosensor toxicity analyser with immobilised Photosystem II (PSII) complex. Using the PSII-biosensor assay, higher sensitivity (LOD) to herbicides (10_8?10_9 M) was achieved as compared to standard algal growth inhibition tests (about 10_7 M). The results showed a good correlation as concerns their EC50 values while the interval of detectable concentrations is about twice wider for PSII-biosensor. Aproposed measurement protocol includes the reference standard of phytotoxicity (RSP). The main advantage of the PSII biosensor assay is that it can be completed in about 1 h and more sensitive than standard algal growth inhibition test.

Název v anglickém jazyce

Detection of photosynthetic herbicides: Algal growth inhibition test vs. electrochemical photosystem II biosensor

Popis výsledku anglicky

We compared a novel PSII-biosensor assay with a standard algal growth inhibition test for detection of photosynthetic herbicides?diuron, atrazine and isoproturon in liquid samples. To evaluate the convenience and sensitivity, values of the parameters EC50 and LOD and the duration of assays were compared. The biosensor assay was made with an electrochemical biosensor toxicity analyser with immobilised Photosystem II (PSII) complex. Using the PSII-biosensor assay, higher sensitivity (LOD) to herbicides (10_8?10_9 M) was achieved as compared to standard algal growth inhibition tests (about 10_7 M). The results showed a good correlation as concerns their EC50 values while the interval of detectable concentrations is about twice wider for PSII-biosensor. Aproposed measurement protocol includes the reference standard of phytotoxicity (RSP). The main advantage of the PSII biosensor assay is that it can be completed in about 1 h and more sensitive than standard algal growth inhibition test.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EH - Ekologie – společenstva

OECD FORD obor

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Projekt

<a href="/cs/project/FT-TA%2F089" target="_blank" >FT-TA/089: IBIS - Inteligentní biosenzorický systém pro detekci pesticidů a herbicidů v životním prostředí.</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Ecotoxicology and Enviromental Safety

ISSN

0147-6513

e-ISSN

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Svazek periodika

74

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000284185300016

EID výsledku v databázi Scopus

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