Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F11%3A00360791" target="_blank" >RIV/61388971:_____/11:00360791 - isvavai.cz</a>
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s00792-011-0373-4" target="_blank" >http://dx.doi.org/10.1007/s00792-011-0373-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00792-011-0373-4" target="_blank" >10.1007/s00792-011-0373-4</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber
Popis výsledku v původním jazyce
Two thermophilic strains belonging to Geobacillus stearothermophilus and Meiothermus ruber, which naturally do not synthesize omega-alicyclic fatty acids (omega-FAs) were cultivated with cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl carboxylic acids. Gas chromatography-mass spectrometry analysis of fatty acid methyl and picolinyl esters showed that both strains are able to synthesize omega-FAs when cultivated with the appropriate precursor. The incorporation of cyclic acids influenced the whole FAcomposition as well as membrane fluidity. Membrane fluidity of intact cells was studied by measuring the fluorescence polarisation of the probe l,6-diphenyl-1,3,5-hexatriene incorporated into membrane lipid bilayers. Cytoplasmic membrane became more fluid with increasing content of omega-FAs. This is caused by considerable changes in lipid packing within the membrane induced by the presence of omega-FAs not found in the natural environment of Geobacillus and Meiothermus strains
Název v anglickém jazyce
Biosynthesis of omega-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria Geobacillus stearothermophilus and Meiothermus ruber
Popis výsledku anglicky
Two thermophilic strains belonging to Geobacillus stearothermophilus and Meiothermus ruber, which naturally do not synthesize omega-alicyclic fatty acids (omega-FAs) were cultivated with cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl carboxylic acids. Gas chromatography-mass spectrometry analysis of fatty acid methyl and picolinyl esters showed that both strains are able to synthesize omega-FAs when cultivated with the appropriate precursor. The incorporation of cyclic acids influenced the whole FAcomposition as well as membrane fluidity. Membrane fluidity of intact cells was studied by measuring the fluorescence polarisation of the probe l,6-diphenyl-1,3,5-hexatriene incorporated into membrane lipid bilayers. Cytoplasmic membrane became more fluid with increasing content of omega-FAs. This is caused by considerable changes in lipid packing within the membrane induced by the presence of omega-FAs not found in the natural environment of Geobacillus and Meiothermus strains
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
EE - Mikrobiologie, virologie
OECD FORD obor
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Návaznosti výsledku
Projekt
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Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2011
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Extremophiles
ISSN
1431-0651
e-ISSN
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Svazek periodika
15
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
JP - Japonsko
Počet stran výsledku
7
Strana od-do
423-429
Kód UT WoS článku
000290037700012
EID výsledku v databázi Scopus
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