Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F11%3A00373580" target="_blank" >RIV/61388971:_____/11:00373580 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985831:_____/11:00373580

Výsledek na webu

<a href="http://dx.doi.org/10.1111/j.1574-6968.2011.02243.x" target="_blank" >http://dx.doi.org/10.1111/j.1574-6968.2011.02243.x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/j.1574-6968.2011.02243.x" target="_blank" >10.1111/j.1574-6968.2011.02243.x</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers

Popis výsledku v původním jazyce

A reliable method of detection in roots and soil is thus needed for assessment of geographic distribution, ecological studies and inoculation efficiency testing in man-made experiments. A PCR-based method of detection of T. aestivum using specific primers was therefore developed. A pair of PCR primers Tu1sekvF/Tu2sekvR selective for T. aestivum and some genotypes of Tuber mesentericum was designed on the basis of the known internal transcribed spacer T. aestivum sequences. TaiI restriction cleavage wasthen used to distinguish the two species. The occurrence of T. aestivum in soil and ectomycorrhizae collected in its native habitat has been successfully detected using the designed primers and nested PCR. The method is reliable and thus suitable for detection of T. aestivum in the field

Název v anglickém jazyce

Detection of summer truffle (Tuber aestivum Vittad.) in ectomycorrhizae and in soil using specific primers

Popis výsledku anglicky

A reliable method of detection in roots and soil is thus needed for assessment of geographic distribution, ecological studies and inoculation efficiency testing in man-made experiments. A PCR-based method of detection of T. aestivum using specific primers was therefore developed. A pair of PCR primers Tu1sekvF/Tu2sekvR selective for T. aestivum and some genotypes of Tuber mesentericum was designed on the basis of the known internal transcribed spacer T. aestivum sequences. TaiI restriction cleavage wasthen used to distinguish the two species. The occurrence of T. aestivum in soil and ectomycorrhizae collected in its native habitat has been successfully detected using the designed primers and nested PCR. The method is reliable and thus suitable for detection of T. aestivum in the field

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP504%2F10%2F0382" target="_blank" >GAP504/10/0382: Tuber aestivum Vitt.: studie rozšíření, biologie a kultivace s perspektivou praktického využití</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FEMS Microbiology Letters

ISSN

0378-1097

e-ISSN

—

Svazek periodika

318

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

84-91

Kód UT WoS článku

000289158900012

EID výsledku v databázi Scopus

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