Tuber aestivum Vittad. mycelium quantified: advantages and limitations of a qPCR approach

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F13%3A00399053" target="_blank" >RIV/61388971:_____/13:00399053 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00572-012-0475-6" target="_blank" >http://dx.doi.org/10.1007/s00572-012-0475-6</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00572-012-0475-6" target="_blank" >10.1007/s00572-012-0475-6</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Tuber aestivum Vittad. mycelium quantified: advantages and limitations of a qPCR approach

Popis výsledku v původním jazyce

A quantitative real-time PCR (qPCR) marker Ta0 with hydrolysis probe ("TaqMan"), targeted to the internal transcribed spacer region of the ribosomal DNA, has been developed for quantification of summer truffle (Tuber aestivum) mycelium. Gene copy concentrations determined by the qPCR were calibrated against pure culture mycelium of T. aestivum, enabling quantification of the mycelium in soil and in host roots from the fields. Significant concentrations of the fungus were observed not only in the finestroots with ectomycorrhizae but also in other root types, indicating that the fungus is an important component of the microbial film at the root surface. The concentration of T. aestivum in soil is relatively high compared to other ectomycorrhizal fungi.To evaluate the reliability of the measurement of the soil mycelium density using qPCR, the steady basal extracellular concentration of the stabilized T. aestivum DNA should be known and taken into account. Therefore, we addressed the sta

Název v anglickém jazyce

Tuber aestivum Vittad. mycelium quantified: advantages and limitations of a qPCR approach

Popis výsledku anglicky

A quantitative real-time PCR (qPCR) marker Ta0 with hydrolysis probe ("TaqMan"), targeted to the internal transcribed spacer region of the ribosomal DNA, has been developed for quantification of summer truffle (Tuber aestivum) mycelium. Gene copy concentrations determined by the qPCR were calibrated against pure culture mycelium of T. aestivum, enabling quantification of the mycelium in soil and in host roots from the fields. Significant concentrations of the fungus were observed not only in the finestroots with ectomycorrhizae but also in other root types, indicating that the fungus is an important component of the microbial film at the root surface. The concentration of T. aestivum in soil is relatively high compared to other ectomycorrhizal fungi.To evaluate the reliability of the measurement of the soil mycelium density using qPCR, the steady basal extracellular concentration of the stabilized T. aestivum DNA should be known and taken into account. Therefore, we addressed the sta

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP504%2F10%2F0382" target="_blank" >GAP504/10/0382: Tuber aestivum Vitt.: studie rozšíření, biologie a kultivace s perspektivou praktického využití</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Mycorrhiza

ISSN

0940-6360

e-ISSN

—

Svazek periodika

23

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

341-348

Kód UT WoS článku

000320329000001

EID výsledku v databázi Scopus

—