In vitro activation of CMV-specific human CD8ţ T cells by adenylate

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F12%3A00377401" target="_blank" >RIV/61388971:_____/12:00377401 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1038/bmt.2011.68" target="_blank" >http://dx.doi.org/10.1038/bmt.2011.68</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/bmt.2011.68" target="_blank" >10.1038/bmt.2011.68</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro activation of CMV-specific human CD8ţ T cells by adenylate

Popis výsledku v původním jazyce

Human CMV infects between 50-85% of healthy individuals and can cause live-threatening infections in immunocompromised patients. Therefore, peptide vaccination is being developed as a promising immunotherapeutic approach for treatment of patients at riskof CMV disease. The enzymatically inactive toxoid of Bordetella adenylate cyclase (CyaA-AC(-)) was shown to be an efficient tool for delivery of peptide epitopes and stimulation of Ag-specific T-cell immune responses. We investigated here the capacity of two CyaA-AC(-) constructs to deliver epitopes derived from the CMV phosphoprotein pp65 for activation of human T cells in vitro. Expansion of gamma-IFN-secreting CMV-specific CD8(+) T cells, as well as increase of total IFN-gamma and TNF-alpha production by PBMCs from CMV-seropositive donors were observed after in vitro stimulation with CyaA-AC(-) constructs carrying CMV epitopes, whereas limited activation of immune response occurred with free peptides

Název v anglickém jazyce

In vitro activation of CMV-specific human CD8ţ T cells by adenylate

Popis výsledku anglicky

Human CMV infects between 50-85% of healthy individuals and can cause live-threatening infections in immunocompromised patients. Therefore, peptide vaccination is being developed as a promising immunotherapeutic approach for treatment of patients at riskof CMV disease. The enzymatically inactive toxoid of Bordetella adenylate cyclase (CyaA-AC(-)) was shown to be an efficient tool for delivery of peptide epitopes and stimulation of Ag-specific T-cell immune responses. We investigated here the capacity of two CyaA-AC(-) constructs to deliver epitopes derived from the CMV phosphoprotein pp65 for activation of human T cells in vitro. Expansion of gamma-IFN-secreting CMV-specific CD8(+) T cells, as well as increase of total IFN-gamma and TNF-alpha production by PBMCs from CMV-seropositive donors were observed after in vitro stimulation with CyaA-AC(-) constructs carrying CMV epitopes, whereas limited activation of immune response occurred with free peptides

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bone Marrow Transplantation

ISSN

0268-3369

e-ISSN

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Svazek periodika

47

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

243-250

Kód UT WoS článku

000300415100013

EID výsledku v databázi Scopus

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