In vitro activation of CMV-specific human CD8(+) T cells by adenylate cyclase toxoids delivering pp65 epitopes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F12%3A%230001441" target="_blank" >RIV/65269705:_____/12:#0001441 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216224:14110/12:00059406 RIV/65269705:_____/12:#0001816

Výsledek na webu

<a href="http://dx.doi.org/10.1038/bmt.2011.68" target="_blank" >http://dx.doi.org/10.1038/bmt.2011.68</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/bmt.2011.68" target="_blank" >10.1038/bmt.2011.68</a>

Jazyk výsledku

angličtina

Název v původním jazyce

In vitro activation of CMV-specific human CD8(+) T cells by adenylate cyclase toxoids delivering pp65 epitopes

Popis výsledku v původním jazyce

Human CMV infects between 50-85% of healthy individuals and can cause live-threatening infections in immunocompromised patients. Therefore, peptide vaccination is being developed as a promising immunotherapeutic approach for treatment of patients at riskof CMV disease. The enzymatically inactive toxoid of Bordetella adenylate cyclase was shown to be an efficient tool for delivery of peptide epitopes and stimulation of Ag-specific T-cell immune responses. We investigated here the capacity of two CyaA-ACconstructs to deliver epitopes derived from the CMV phosphoprotein pp65 for activation of human T cells in vitro. Expansion of IFN-secreting CMV-specific CD8(+) T cells, as well as increase of total production by PBMCs from CMV-seropositive donors wereobserved after in vitro stimulation with CyaA-AC( constructs carrying CMV epitopes, whereas limited activation of immune response occurred with free peptides. The activation of immune response was confirmed by expansion of CMV-specific T-

Název v anglickém jazyce

In vitro activation of CMV-specific human CD8(+) T cells by adenylate cyclase toxoids delivering pp65 epitopes

Popis výsledku anglicky

Human CMV infects between 50-85% of healthy individuals and can cause live-threatening infections in immunocompromised patients. Therefore, peptide vaccination is being developed as a promising immunotherapeutic approach for treatment of patients at riskof CMV disease. The enzymatically inactive toxoid of Bordetella adenylate cyclase was shown to be an efficient tool for delivery of peptide epitopes and stimulation of Ag-specific T-cell immune responses. We investigated here the capacity of two CyaA-ACconstructs to deliver epitopes derived from the CMV phosphoprotein pp65 for activation of human T cells in vitro. Expansion of IFN-secreting CMV-specific CD8(+) T cells, as well as increase of total production by PBMCs from CMV-seropositive donors wereobserved after in vitro stimulation with CyaA-AC( constructs carrying CMV epitopes, whereas limited activation of immune response occurred with free peptides. The activation of immune response was confirmed by expansion of CMV-specific T-

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Bone Marrow Transplantation

ISSN

0268-3369

e-ISSN

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Svazek periodika

47

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

243-250

Kód UT WoS článku

000300415100013

EID výsledku v databázi Scopus

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