Structural analysis of mycolic acids from phenol-degrading strain of Rhodococcus erythropolis by liquid chromatography-tandem mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388971%3A_____%2F12%3A00387053" target="_blank" >RIV/61388971:_____/12:00387053 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22340/12:43893286 RIV/60461373:22330/12:43893286

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s12223-012-0156-z" target="_blank" >http://dx.doi.org/10.1007/s12223-012-0156-z</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-012-0156-z" target="_blank" >10.1007/s12223-012-0156-z</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Structural analysis of mycolic acids from phenol-degrading strain of Rhodococcus erythropolis by liquid chromatography-tandem mass spectrometry

Popis výsledku v původním jazyce

We used reversed phase liquid chromatography-electrospray ionization tandem mass spectrometry for direct analysis of mycolic acids (MAs) from four different cultivations of Rhodococcus erythropolis. This technique enabled us to identify and quantify thespecific molecular species of MAs directly from lipid extracts of the bacterium, including the determination of their basic characteristics such as retention time and mass spectra. We identified a total of 60 molecular species of MAs by means of LC/MS. In collision-induced dissociation tandem mass spectrometry, the [M-H](-) ions eliminated two residues, i.e., meroaldehyde and carboxylate anions containing alpha-alkyl chains. The structural information from these fragment ions affords structural assignment of the mycolic acids, including the lengths and number of double bond(s). Two strains, i.e., R. erythropolis CCM 2595 and genetically modified strain CCM 2595 pSRK 21 phe were cultivated on two different substrates (phenol and phenol w

Název v anglickém jazyce

Structural analysis of mycolic acids from phenol-degrading strain of Rhodococcus erythropolis by liquid chromatography-tandem mass spectrometry

Popis výsledku anglicky

We used reversed phase liquid chromatography-electrospray ionization tandem mass spectrometry for direct analysis of mycolic acids (MAs) from four different cultivations of Rhodococcus erythropolis. This technique enabled us to identify and quantify thespecific molecular species of MAs directly from lipid extracts of the bacterium, including the determination of their basic characteristics such as retention time and mass spectra. We identified a total of 60 molecular species of MAs by means of LC/MS. In collision-induced dissociation tandem mass spectrometry, the [M-H](-) ions eliminated two residues, i.e., meroaldehyde and carboxylate anions containing alpha-alkyl chains. The structural information from these fragment ions affords structural assignment of the mycolic acids, including the lengths and number of double bond(s). Two strains, i.e., R. erythropolis CCM 2595 and genetically modified strain CCM 2595 pSRK 21 phe were cultivated on two different substrates (phenol and phenol w

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

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Svazek periodika

57

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

11

Strana od-do

473-483

Kód UT WoS článku

000310087200001

EID výsledku v databázi Scopus

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